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beta-Site APP-Cleaving Enzyme 2 (BACE2) (C-Term) antibody

Details for Product No. ABIN499397, Supplier: Log in to see
Antigen
  • 1110059C24Rik
  • AEPLC
  • AI850424
  • ALP56
  • ARP1
  • ASP1
  • ASP21
  • BAE2
  • CDA13
  • CEAP1
  • DRAP
Epitope
C-Term
32
20
15
15
14
10
5
5
5
4
3
3
2
1
1
1
1
1
1
1
1
1
1
1
1
Reactivity
Human
143
49
47
Host
Rabbit
151
2
1
Clonality
Polyclonal
Conjugate
Un-conjugated
9
8
4
4
4
3
2
1
1
1
1
1
1
1
1
Application
Enzyme Immunoassay (EIA), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)
121
75
41
26
17
12
7
6
3
3
2
Supplier
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Immunogen Human BACE2 (C-Terminus) Peptide
Isotype IgG
Specificity BACE2 antibody was raised against a peptide corresponding to amino acids near the carboxy terminus of human BACE2.
Purification Affinity chromatography purified via peptide column
Alternative Name BACE2 (BACE2 Antibody Abstract)
Background Accumulation of the amyloid-beta (alphabetaβ) plaque in the cerebral cortex is a critical event in the pathogenesis of Alzheimer's disease. alphabeta peptide is generated by proteolytic cleavage of the beta-amyloid protein precursor (APP) at beta- and gamma-sites by proteases. The long-sought beta-secretase was recently identified by several groups independently and designated beta-site APP cleaving enzyme (BACE) and aspartyl protease 2 (Asp2) (1-4). BACE/Asp2 is a novel transmembrane aspartic protease and co-localizes with APP. A BACE homolog was recently cloned and designated BACE2, Asp1, DRAP (for Down region aspartic protease), and memapsin 1 (4-9). BACE2 also cleaves APP at b-site and at a different site within alphabeta (8). BACE2 locates on chromosome 21q22.3, the so-called 'Down critical region', suggesting that BACE2 and alphabeta may also contribute to the pathogenesis of Down syndrome (6,7)Synonyms: AEPLC, ALP56, ASP1, ASP21, Aspartic-like protease 56 kDa, Aspartyl protease 1, Beta-secretase 2, Beta-site APP-cleaving enzyme 2, Down region aspartic protease, Memapsin-1, Membrane-associated aspartic protease 1
Gene ID 25825
UniProt Q9Y5Z0
Research Area Proteolysis / Ubiquitin, Alzheimer's Disease, Enzymes
Application Notes ELISA. Western Blot: BACE2 antibody can be used for detection of BACE2 at 0.5 to 1 μg/mL. Immunohistochemistry.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.
Restrictions For Research Use only
Buffer PBS containing 0.02 % sodium azide.
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage 4 °C
Storage Comment Store the antibody undiluted at 2-8 °C.
Supplier Images
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) image for anti-beta-Site APP-Cleaving Enzyme 2 (BACE2) (C-Term) antibody (ABIN499397) Immunohistochemistry of BACE2 in rat heart tissue with BACE2 antibody at 2 μg/ml.
Western Blotting (WB) image for anti-beta-Site APP-Cleaving Enzyme 2 (BACE2) (C-Term) antibody (ABIN499397) Western blot analysis of BACE2 in human heart tissue lysate in the absence (A) or pre...
Background publications Hussain, Powell, Howlett, Tew, Meek, Chapman, Gloger, Murphy, Southan, Ryan, Smith, Simmons, Walsh, Dingwall, Christie: "Identification of a novel aspartic protease (Asp 2) as beta-secretase." in: Molecular and cellular neurosciences, Vol. 14, Issue 6, pp. 419-27, 2000 (PubMed).

Vassar, Bennett, Babu-Khan, Kahn, Mendiaz, Denis, Teplow, Ross, Amarante, Loeloff, Luo, Fisher, Fuller, Edenson, Lile, Jarosinski, Biere, Curran, Burgess, Louis, Collins, Treanor, Rogers, Citron: "Beta-secretase cleavage of Alzheimer's amyloid precursor protein by the transmembrane aspartic protease BACE." in: Science (New York, N.Y.), Vol. 286, Issue 5440, pp. 735-41, 1999 (PubMed).

Sinha, Anderson, Barbour, Basi, Caccavello, Davis, Doan, Dovey, Frigon, Hong, Jacobson-Croak, Jewett, Keim, Knops, Lieberburg, Power, Tan, Tatsuno, Tung, Schenk, Seubert, Suomensaari, Wang, Walker et al.: "Purification and cloning of amyloid precursor protein beta-secretase from human brain. ..." in: Nature, Vol. 402, Issue 6761, pp. 537-40, 1999 (PubMed).

Yan, Bienkowski, Shuck, Miao, Tory, Pauley, Brashier, Stratman, Mathews, Buhl, Carter, Tomasselli, Parodi, Heinrikson, Gurney: "Membrane-anchored aspartyl protease with Alzheimer's disease beta-secretase activity." in: Nature, Vol. 402, Issue 6761, pp. 533-7, 1999 (PubMed).