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Checkpoint Kinase 2 (CHEK2) (N-Term) antibody

Details for Product No. ABIN499619, Supplier: Log in to see
Antigen
  • CHK-2
  • CDS1
  • CHK2
  • HuCds1
  • LFS2
  • PP1425
  • RAD53
  • hCds1
  • Cds1
  • HUCDS1
  • Rad53
  • Chk2
  • fa66f08
  • wu:fa66f08
  • zgc:55865
Alternatives
anti-Human Checkpoint Kinase 2 antibody for Enzyme Immunoassay
Epitope
N-Term
45
41
38
32
24
15
13
13
12
11
10
9
7
7
6
5
3
3
3
3
3
3
3
3
3
3
3
2
2
2
2
2
2
2
2
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
Reactivity
Human, Mouse (Murine), Rat (Rattus)
404
161
160
10
8
7
7
7
5
1
1
1
Host
Rabbit
325
85
8
1
Clonality
Polyclonal
Conjugate
Un-conjugated
7
7
6
6
5
5
5
5
5
5
5
5
1
1
1
1
1
Application
Enzyme Immunoassay (EIA), Immunofluorescence (IF), Western Blotting (WB)
322
114
112
63
61
57
37
24
21
6
4
2
1
Supplier
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Immunogen Human Chk2 (N-Terminus) Peptide
Isotype IgG
Specificity Chk2 antibody was raised against a synthetic peptide corresponding to amino acids near the amino terminus of human Chk2.
Purification Affinity chromatography purified via peptide column
Alternative Name CHK2 (CHEK2 Antibody Abstract)
Background The p53 tumor-suppressor gene integrates numerous signals that control cell life and death. Several novel molecules involved in p53 signaling, including Chk2 , p53R2 (2), p53AIP1 (3), Noxa (4), PIDD (5), and PID/MTA2 (6), were recently discovered. The checkpoint kinase Chk2 is the mammalian homologue of yeast Cds1/Rad53. In response to DNA damage, the checkpoint kinase ATM phosphorylates and activates Chk2, which in turn directly phosphorylates and activates p53 (7,8). Chk2 serves as ATM downstream effector to mediate activation of p53. Chk2 also phosphorylates and activates BRCA1, the product of a tumor suppressor gene that is mutated in breast and ovarian cancer (9).Synonyms: CHEK-2, CHEK2, CHK-2, CHK2 checkpoint homolog, Cds1, RAD53, Serine/threonine-protein kinase Chk2
Gene ID 11200
NCBI Accession NP_009125
UniProt O96017
Pathways p53 Signaling, Apoptosis, Cell Division Cycle
Application Notes ELISA. Western Blot: Chk2 antibody can be used for detection of Chk2 at 0.5 to 1 μg/mL. A 60 kDaband can be detected. Immunocytochemistry.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.
Restrictions For Research Use only
Concentration 1 mg/mL
Buffer PBS containing 0.02 % sodium azide.
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage 4 °C
Storage Comment Store the antibody undiluted at 2-8 °C.
Supplier Images
Immunofluorescence (IF) image for anti-Checkpoint Kinase 2 (CHEK2) (N-Term) antibody (ABIN499619) Immunocytochemistry of Chk2 in Jurkat cells with AP30226PU-N Chk2 antibody at 1 μg/ml.
Western Blotting (WB) image for anti-Checkpoint Kinase 2 (CHEK2) (N-Term) antibody (ABIN499619) Western blot analysis of Chk2 expression in K562 (A), Jurkat (B), and HL-60 (C) whole...
Background publications Oda, Arakawa, Tanaka et al.: "p53AIP1, a potential mediator of p53-dependent apoptosis, and its regulation by Ser-46-phosphorylated p53." in: Cell, Vol. 102, Issue 6, pp. 849-62, 2000 (PubMed).

Oda, Ohki, Murasawa et al.: "Noxa, a BH3-only member of the Bcl-2 family and candidate mediator of p53-induced apoptosis." in: Science (New York, N.Y.), Vol. 288, Issue 5468, pp. 1053-8, 2000 (PubMed).

Tanaka, Arakawa, Yamaguchi et al.: "A ribonucleotide reductase gene involved in a p53-dependent cell-cycle checkpoint for DNA damage." in: Nature, Vol. 404, Issue 6773, pp. 42-9, 2000 (PubMed).

Matsuoka, Huang, Elledge: "Linkage of ATM to cell cycle regulation by the Chk2 protein kinase." in: Science (New York, N.Y.), Vol. 282, Issue 5395, pp. 1893-7, 1998 (PubMed).