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Integrin, alpha 4 (Antigen CD49D, alpha 4 Subunit of VLA-4 Receptor) (ITGA4) (C-Term) antibody

Details for Product No. ABIN500028, Supplier: Log in to see
Antigen
  • LOC100219853
  • CD49D
  • IA4
Epitope
C-Term
21
5
4
4
4
4
3
3
2
2
1
1
1
1
1
1
1
1
1
1
Reactivity
Human, Mouse (Murine), Rat (Rattus)
263
213
67
11
8
8
7
6
4
4
4
3
3
3
2
1
1
1
1
Host
Rabbit
203
189
71
Clonality
Polyclonal
Conjugate
Un-conjugated
63
51
29
8
6
4
4
4
1
1
1
1
Application
Enzyme Immunoassay (EIA), Western Blotting (WB)
369
123
89
70
48
31
30
29
23
18
9
6
3
3
3
3
2
2
2
2
2
2
1
Supplier
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Immunogen Integrin alpha 4 antibody was raised against a 13 amino acid peptide from near the carboxy terminus of human Integrin alpha 4.
Isotype IgG
Specificity This antibody reacts to Integrin alpha 4.
Purification Affinity chromatography purified via peptide column
Alternative Name CD49d / ITGA4 (ITGA4 Antibody Abstract)
Background The integrin alpha 4 (also known as CD49d and ITGA4) belongs to the integrin alpha chain family of proteins. Integrins are heterodimeric integral membrane proteins composed of an alpha and beta chains. Alpha 4 chain associates with either beta 1 or beta 7 chain. It has been demonstrated that the putative ligand-binding sites of both integrin alpha 4 beta 1 and alpha 4 beta 7 is located on the alpha 4 chain. These ligands included Madcam, VCAM, and fibronectin. Madcam is known as the principal ligand for integrin alpha 4 beta 7. Recently it was also demonstrated that HIV-1 envelope can mimic Madcam by binding to and signaling though integrin alpha 4 beta 7, the gut mucosal homing receptor for peripheral T cells. Despite its predicted molecular weight, Integrin alpha 4 will often run at higher molecular weight in SDS-PAGE. At least two isoforms are known to exist. The immunogen to this antibody is outside of the Integrin alpha 4 recombinant protein fragment and thus will not recognize it.Synonyms: CD49 antigen-like family member D, Integrin alpha-4, Integrin alpha-IV, VLA-4, VLA4
Gene ID 3676
UniProt P13612
Research Area CD Antigens, Surface Receptors of Immune Cells, Cell/Tissue Markers, Extracellular Matrix, Signaling
Application Notes ELISA. Western Blot: 0.5 - 1 μg/mL.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.
Restrictions For Research Use only
Buffer PBS containing 0.02 % sodium azide.
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage 4 °C
Storage Comment Store the antibody undiluted at 2-8 °C.
Supplier Images
Western Blotting (WB) image for anti-Integrin, alpha 4 (Antigen CD49D, alpha 4 Subunit of VLA-4 Receptor) (ITGA4) (C-Term) antibody (ABIN500028) Western blot analysis of Integrin alpha 4 in Jurkat cell lysate with AP30434PU-N Inte...
Background publications Wegener, Campbell: "Transmembrane and cytoplasmic domains in integrin activation and protein-protein interactions (review)." in: Molecular membrane biology, Vol. 25, Issue 5, pp. 376-87, 2008 (PubMed).

Berlin, Berg, Briskin et al.: "Alpha 4 beta 7 integrin mediates lymphocyte binding to the mucosal vascular addressin MAdCAM-1." in: Cell, Vol. 74, Issue 1, pp. 185-95, 1993 (PubMed).

Kamata, Puzon, Takada: "Identification of putative ligand-binding sites of the integrin alpha 4 beta 1 (VLA-4, CD49d/CD29)" in: The Biochemical journal, Vol. 305 ( Pt 3), pp. 945-51, 1995 (PubMed).

Pulido, Elices, Campanero et al.: "Functional evidence for three distinct and independently inhibitable adhesion activities mediated by the human integrin VLA-4. Correlation with distinct alpha 4 epitopes." in: The Journal of biological chemistry, Vol. 266, Issue 16, pp. 10241-5, 1991 (PubMed).