NLRC4 antibody

Catalog No. ABIN500034

Product Details anti-NLRC4 Antibody

Target: NLRC4 (NLR Family, CARD Domain Containing 4 (NLRC4))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This NLRC4 antibody is un-conjugated

Application: Western Blotting (WB), Immunofluorescence (IF), Enzyme Immunoassay (EIA)

Specificity: Ipaf antibody was raised against a synthetic peptide corresponding to amino acids near the C-terminus of human Ipaf.

Purification: Affinity chromatography purified via peptide column

Immunogen: Human Ipaf / CARD12 / CLANA Peptide

Isotype: IgG

Application Details

Application Notes: ELISA. Western Blot: 0.5 to 2 μg/mL. Human peripheral blood leukocyte (PBL) cell lysate can beused as positive control and a 110 kDa band can be detected. Immunocytochemistry.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Buffer: PBS containing 0.02 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C

Storage Comment: Store the antibody undiluted at 2-8 °C.

Target Details for NLRC4

Target: NLRC4 (NLR Family, CARD Domain Containing 4 (NLRC4))

Alternative Name: NLRC4 (NLRC4 Products)

Synonyms: CARD12 antibody, CLAN antibody, CLAN1 antibody, CLANA antibody, CLANB antibody, CLANC antibody, CLAND antibody, CLR2.1 antibody, IPAF antibody, 9530011P19Rik antibody, Card12 antibody, NLR family CARD domain containing 4 antibody, NLR family, CARD domain containing 4 antibody, NLRC4 antibody, Nlrc4 antibody

Background: Apoptosis is related to many diseases and induced by a family of cell death receptors and their ligands. Cell death signals are transduced by death domain containing adaptor molecules and proteases including several members of the caspase family. Another family of proteins that functions as a critical regulator of apoptosis and NF-kappaB signaling pathways is the CED-4/Apaf-1 (apoptosis protein activating factor-1) protein family (1). Ipaf (ICE protease activating factor) is a CED-4/Apaf-1 family member that activates caspase-1/ICE and can induce apoptosis in human cells in a caspase-1 dependent manner (2,3). Ipaf and caspase-1 are thought to interact with each other through the association of the Ipaf amino-terminal CARD (caspase recruitment domain) and amino-terminal CARD of caspase-1.Synonyms: CARD12, CLAN, CLAN1, IPAF, NLR family CARD domain-containing protein 4

Gene ID: 58484

NCBI Accession: NP_067032

UniProt: Q9NPP4

Pathways: Activation of Innate immune Response, Positive Regulation of Endopeptidase Activity, Inflammasome