IRF3 antibody (Intermediate Domain)

Catalog No. ABIN500056

Product Details anti-IRF3 Antibody

Target: IRF3 (Interferon Regulatory Factor 3 (IRF3))

Binding Specificity: Intermediate Domain

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This IRF3 antibody is un-conjugated

Application: Western Blotting (WB), Immunofluorescence (IF), Enzyme Immunoassay (EIA)

Specificity: IRF3 antibody antibody was raised against a peptide corresponding to 14 amino acids near the center of human IRF3.

Purification: Affinity chromatography purified via peptide column

Immunogen: Human IRF3 (Intermediate Domain) Peptide

Isotype: IgG

Application Details

Application Notes: ELISA. Western Blot: 1 - 2 μg/mL. Immunocytochemistry.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Buffer: PBS containing 0.02 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C

Storage Comment: Store the antibody undiluted at 2-8 °C.

Target Details for IRF3

Target: IRF3 (Interferon Regulatory Factor 3 (IRF3))

Alternative Name: IRF3 (IRF3 Products)

Synonyms: irf3 antibody, IRF3 antibody, C920001K05Rik antibody, IRF-3 antibody, interferon regulatory factor 3 antibody, interferon regulatory factor 7 antibody, irf3 antibody, IRF3 antibody, irf-3 antibody, Irf3 antibody, IRF7 antibody

Background: Interferons (IFN)s are involved in a multitude of immune interactions during viral infections and play a major role in both the induction and regulation of innate and adaptive antiviral mechanisms (1 for review). During infection, host-virus interactions signal downstream molecules such as transcription factors such as IFN regulatory factor-3 (IRF3) which can act to stimulate transcription of IFN-alpha/beta genes. IRF3 is present in an inactive form in the cytoplasm of most cells (2). Following viral infection, IRF3 can be activated by IkappaB kinase-epsilon and TANK-binding kinase 1 (TBK1) (3,4), whereupon IRF3 translocates to the nucleus. IRF3 can also be activated by stimulation of toll-like receptor 3 (TLR3) by dsRNA (3,5). IRF3 exists as at least two distinct isoforms.Synonyms: IRF-3, Interferon regulatory factor 3

Gene ID: 3661

NCBI Accession: NP_001562

UniProt: Q14653

Pathways: TLR Signaling, Activation of Innate immune Response, Cellular Response to Molecule of Bacterial Origin, Hepatitis C, Toll-Like Receptors Cascades