You are viewing an incomplete version of our website. Please click to reload the website as full version.

Receptor-Interacting serine-threonine Kinase 3 (RIPK3) (AA 473-486) antibody

Details for Product No. ABIN500612, Supplier: Log in to see
Antigen
  • RIPK3
  • RIP3
  • 2610528K09Rik
  • AW107945
  • Rip3
  • Hcyp2
Epitope
AA 473-486
32
20
16
15
10
6
5
5
4
3
3
2
2
2
2
2
2
2
2
1
1
1
Reactivity
Mouse (Murine), Rat (Rattus)
115
39
15
7
6
4
2
1
Host
Rabbit
121
23
Clonality
Polyclonal
Conjugate
Un-conjugated
9
7
5
5
5
5
1
Application
Enzyme Immunoassay (EIA), Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)
128
61
60
22
7
3
2
2
1
1
1
1
Supplier
Log in to see
Supplier Product No.
Log in to see
Request

Showcase your results, aid the scientific community, and receive a full refund.

Contribute a validation

Learn more

Available images

Immunogen RIP3 antibody was raised against a peptide corresponding to amino acids 473 to 486 of Murine RIP3.
Isotype IgG
Specificity This antibody detects RIP3.
Cross-Reactivity (Details) Species reactivity (tested):Mouse, Rat.
Purification Peptide Affinity Chromatography
Alternative Name RIPK3 / RIP3 (RIPK3 Antibody Abstract)
Background Certain serine/threonine protein kinases, such as ASK1, RIP, DAP, and ZIP kinases, are mediators of apoptosis. Receptor interacting proteins including RIP and RIP2/RICK mediate apoptosis induced by TNFR1 and Fas, two prototype members in the death receptor family. A novel member in the RIP kinase family was recently identified and designated RIP3 (1-3). RIP3 contains N-terminal kinase domain but, unlike RIP or RIP2, lacks the C-terminal death or CARD domain. RIP3 binds to RIP and TNFR1, mediates TNFR1 induced apoptosis, and attenuates RIP and TNFR1 induced NF-kB activation. Overexpression of RIP3 induces apoptosis and NF-kappaB activation. The messenger RNA of RIP3 is expressed in a subset of adult tissues (2,3).Synonyms: RIP-3, RIP-like protein kinase 3, Receptor-interacting protein 3, Receptor-interacting serine/threonine-protein kinase 3
Molecular Weight 57 kDa
Gene ID 56532
UniProt Q9QZL0
Pathways
Application Notes ELISA. Western blot: 0.5 to 1 μg/mL. Immunohistochemistry on paraffin sections.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.
Restrictions For Research Use only
Concentration 1.0 mg/mL
Buffer PBS, 0.02 % Sodium Azide
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Avoid repeated freezing and thawing.
Storage 4 °C/-20 °C
Storage Comment Store undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.
Supplier Images
Immunofluorescence (IF) image for anti-Receptor-Interacting serine-threonine Kinase 3 (RIPK3) (AA 473-486) antibody (ABIN500612) Immunofluorescence of RIP3 in Rat Kidney cells with RIP3 antibody at 20 µg/ml.
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) image for anti-Receptor-Interacting serine-threonine Kinase 3 (RIPK3) (AA 473-486) antibody (ABIN500612) Immunohistochemistry of RIP3 in rat kidney tissue with RIP3 antibody at 5 μg/ml.
Western Blotting (WB) image for anti-Receptor-Interacting serine-threonine Kinase 3 (RIPK3) (AA 473-486) antibody (ABIN500612) Western blot analysis of RIP3 in mouse 3T3 whole cell lysate in the absence (A) or pr...
Background publications Pazdernik, Donner, Goebl et al.: "Mouse receptor interacting protein 3 does not contain a caspase-recruiting or a death domain but induces apoptosis and activates NF-kappaB." in: Molecular and cellular biology, Vol. 19, Issue 10, pp. 6500-8, 2000 (PubMed).

Sun, Lee, Navas et al.: "RIP3, a novel apoptosis-inducing kinase." in: The Journal of biological chemistry, Vol. 274, Issue 24, pp. 16871-5, 1999 (PubMed).

Yu, Huang, Shen et al.: "Identification of RIP3, a RIP-like kinase that activates apoptosis and NFkappaB." in: Current biology : CB, Vol. 9, Issue 10, pp. 539-42, 1999 (PubMed).