You are viewing an incomplete version of our website. Please click to reload the website as full version.

TNFSF12 antibody (Tumor Necrosis Factor (Ligand) Superfamily, Member 12)

Details for Product anti-TNFSF12 Antibody No. ABIN501094, Supplier: Log in to see
Antigen
  • APO3L
  • Apo3l
  • Dr3l
  • Dr3lg
  • DR3LG
  • Tweak
  • TWEAK
Alternatives
anti-Human TNFSF12 antibody for Enzyme Immunoassay
Reactivity
Human, Mouse (Murine), Rat (Rattus)
125
32
11
3
1
Host
Rabbit
70
39
18
8
Clonality
Polyclonal
Conjugate
This TNFSF12 antibody is un-conjugated
11
5
4
3
2
2
1
1
1
1
1
1
1
1
1
1
1
1
1
1
Application
Enzyme Immunoassay (EIA), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)
101
69
43
30
25
23
10
10
4
4
2
2
1
1
Supplier
Log in to see
Supplier Product No.
Log in to see
Request

Get this product for free

It's quick and easy to submit your validation proposal. I want to validate this product

Learn more

Available images

Immunogen Recombinant Human TWEAK.
Isotype IgG
Specificity This antibody detects TWEAK / TNFSF12.
Cross-Reactivity (Details) Species reactivity (tested):Human, Mouse, Rat.
Purification Peptide Affinity Chromatography
Alternative Name TWEAK / TNFSF12 (TNFSF12 Antibody Abstract)
Background TNF-related weak inducer of apoptosis (TWEAK) is a member of the tumor necrosis factor superfamily (TNFSF) of structurally related cytokines. Like most other members of this family, TWEAK is a cell surface-associated type II transmembrane protein although a smaller, biologically active form can also be generated by cleavage near the cell membrane (1,2). TWEAK has multiple biological activities, including stimulation of cell growth and angiogenesis (3), induction of inflammatory cytokines (4), in addition to stimulation of apoptosis (5). The TWEAK signal transduction pathway has not been well established but it appears to signal via TweakR/Fn14 in a manner similar to that described for other TNFSF members that bind receptors lacking death domains (1). Despite its predicted molecular weight of 27 kDa, TWEAK may migrate at aberrant positions in SDS-PAGE.Synonyms: APO3 ligand, APO3L, DR3LG, TNF-related weak inducer of apoptosis, Tumor necrosis factor ligand superfamily member 12
Gene ID 8742
NCBI Accession NP_003800
Pathways Apoptosis
Application Notes ELISA. Western blot: 0.5 - 1 μg/mL. Immunohistochemistry on paraffin sections.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.
Restrictions For Research Use only
Concentration 1.0 mg/mL
Buffer PBS containing 0.02 % Sodium Azide as preservative
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Avoid repeated freezing and thawing.
Storage 4 °C/-20 °C
Storage Comment Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.
Supplier Images
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) image for anti-TNFSF12 antibody (Tumor Necrosis Factor (Ligand) Superfamily, Member 12) (ABIN501094) Immunohistochemistry of TWEAK in human brain tissue with this product at 10 μg/ml.
Western Blotting (WB) image for anti-TNFSF12 antibody (Tumor Necrosis Factor (Ligand) Superfamily, Member 12) (ABIN501094) Western blot analysis of (A) 5 ng, (B) 25 ng and (C) 50 ng of recombinant TWEAK with ...
Background publications Kaplan, Lewis, Shelden, Somers, Pavlic, McCune, Richardson: "The apoptotic ligands TRAIL, TWEAK, and Fas ligand mediate monocyte death induced by autologous lupus T cells." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 169, Issue 10, pp. 6020-9, 2002 (PubMed).

Chicheportiche, Chicheportiche, Sizing, Thompson, Benjamin, Ambrose, Dayer: "Proinflammatory activity of TWEAK on human dermal fibroblasts and synoviocytes: blocking and enhancing effects of anti-TWEAK monoclonal antibodies." in: Arthritis research, Vol. 4, Issue 2, pp. 126-33, 2002 (PubMed).

Yoshida, Matsui, Yamamoto, Okada, Mori: "XBP1 mRNA is induced by ATF6 and spliced by IRE1 in response to ER stress to produce a highly active transcription factor." in: Cell, Vol. 107, Issue 7, pp. 881-91, 2002 (PubMed).

Lynch, Wang, Lund, Chen, Leal, Wiley: "TWEAK induces angiogenesis and proliferation of endothelial cells." in: The Journal of biological chemistry, Vol. 274, Issue 13, pp. 8455-9, 1999 (PubMed).

Chicheportiche, Bourdon, Xu, Hsu, Scott, Hession, Garcia, Browning: "TWEAK, a new secreted ligand in the tumor necrosis factor family that weakly induces apoptosis." in: The Journal of biological chemistry, Vol. 272, Issue 51, pp. 32401-10, 1998 (PubMed).