UBE2N / BLU (C-Term) antibody

Details for Product No. ABIN501096
Request Want additional data for this product?

The Independent Validation Initiative strives to provide you with high quality data. Find out more

Antigen
Epitope
C-Term
(3), (2)
Reactivity
Human, Mouse (Murine), Rat (Rattus)
(9), (8), (8), (3), (3), (3), (3), (2), (2), (2), (1), (1)
Host
Rabbit
(5), (3), (1)
Clonality
Polyclonal
Application
ELISA, Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)
(9), (6), (4), (2), (2), (2), (1)
Pubmed 4 references available
Catalog no. ABIN501096
Quantity 0.1 mg
Price
401.50 $   Plus shipping costs $45.00
Shipping to United States (Change)
Availability Will be delivered in 6 to 8 Business Days

Order hotline:

  • +1 404 474 4654
  • +1 888 205 9894 (TF)
Immunogen UBC13 antibody was raised against a peptide corresponding to 15 amino acids near the C- terminus of human UBC13.
Isotype IgG
Specificity This antibody detects UBE2N / BLU.
Purification Ion exchange chromatography
Background Ubiquitin-conjugating enzyme 13 (Ubc13) was initially discovered in S. cerevisiae as a DNA- damage inducible protein involved in the error-free DNA postreplication repair pathway (1). It has recently been shown to be an important component of the Toll-like receptor and IL-1R signaling pathway (reviewed in 2). Signals from these pathways are relayed by a number of downstream molecules such as MyD88 and tumor necrosis factor receptor associated factor (TRAF6), ultimately activating various kinases and transcription factors (2,3). Ubc13 is part of a dimeric ubiquitin-conjugating enzyme complex also containing UEV1A (ubiquitin- conjugating enzyme E2 variant 1) that together with TRAF6 activates TAK1, a member of the mitogen-activated protein kinase kinase kinase family (4-6). The Ubc13-UEV1A complex also mediates the Lys-63 ubiquitination of TRAF-6, and this ubiquitination is essential for TAK1 activation (5).
Alternate names: Bendless-like ubiquitin-conjugating enzyme, UBC13, Ubiquitin carrier protein N, Ubiquitin- conjugating enzyme E2 N, Ubiquitin-protein ligase N
Gene ID 7334
NCBI Accession AAP35519
Application Notes ELISA. Western blot. Immunohistochemistry on paraffin sections. Other applications not tested. Optimal dilutions are dependent on conditions and should be determined by the user.
Restrictions For Research Use only
Format Liquid
Buffer PBS containing 0.02% sodium azide
Preservative Sodium azide
Handling Advice Avoid repeated freezing and thawing.
Storage 4 °C/-20 °C
Storage Comment Store at 2 - 8 °C for up to one month or (in aliquots) at -20 °C for longer.
Expiry Date 12 months
Background publications Brusky, Zhu, Xiao: "UBC13, a DNA-damage-inducible gene, is a member of the error-free postreplication repair pathway in Saccharomyces cerevisiae." in: Current genetics, Vol. 37, Issue 3, pp. 168-74, 2000 (PubMed).

Deng, Wang, Spencer et al.: "Activation of the IkappaB kinase complex by TRAF6 requires a dimeric ubiquitin-conjugating enzyme complex and a unique polyubiquitin chain." in: Cell, Vol. 103, Issue 2, pp. 351-61, 2000 (PubMed).

Vogel, Fitzgerald, Fenton: "TLRs: differential adapter utilization by toll-like receptors mediates TLR-specific patterns of gene expression." in: Molecular interventions, Vol. 3, Issue 8, pp. 466-77, 2004 (PubMed).

Akira, Takeda: "Toll-like receptor signalling." in: Nature reviews. Immunology, Vol. 4, Issue 7, pp. 499-511, 2004 (PubMed).

Hosts (5), (3), (1)
Reactivities (9), (8), (8), (3), (3), (3), (3), (2), (2), (2), (1), (1)
Applications (9), (6), (4), (2), (2), (2), (1)
Epitopes (3), (2)
Request Want additional data for this product?

The Independent Validation Initiative strives to provide you with high quality data. Find out more

Order hotline:

  • +1 404 474 4654
  • +1 888 205 9894 (TF)
Validation Images
back to top