Mitogen-Activated Protein Kinase Kinase 3 (MAP2K3) (C-Term) antibody

Details for Product No. ABIN502212
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Antigen
Synonyms Mkk3, MAPKK3, MEK3, MKK3, PRKMK3, SAPKK-2, SAPKK2, AW212142, Prkmk3, mMKK3b, map2k3, zMKK3
Epitope
C-Term
(42), (22), (12), (8), (7), (5), (4), (4), (4), (3), (3), (3), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Reactivity
Mouse (Murine), Rat (Rattus), Cow (Bovine), Dog (Canine), Zebrafish (Danio rerio), Xenopus laevis, Human, Chicken
(198), (103), (94), (25), (24), (14), (12), (12), (1), (1)
Host
Rabbit
(162), (37)
Clonality
Polyclonal
Conjugate
Un-conjugated
(4), (4), (4), (3), (3), (3), (3), (3), (3), (3), (3), (1), (1), (1), (1), (1)
Application
Western Blotting (WB)
(166), (69), (54), (47), (42), (30), (13), (11), (3), (1), (1), (1), (1)
Pubmed 1 reference available
Quantity 50 μg
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Catalog No. ABIN502212
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Immunogen Synthetic peptide directed towards the C terminal of human MAP2K3
Sequence EEPSPQLPAD RFSPEFVDFT AQCLRKNPAE RMSYLELMEH PFFTLHKTKK
Predicted Reactivity Bovine : 100 %, Dog : 100 %, Guinea pig : 100 %, Horse : 100 %, Human : 100 %, Rabbit : 100 %, Mouse : 90 %, Rat : 90 %, African clawed frog : 80 %, Chicken : 70 %
Characteristics This is a rabbit polyclonal antibody against MAP2K3. It was validated on Western Blot using a cell lysate as a positive control.
Purification Affinity Purified
Alternative Name MAP2K3
Background MAP2K3 is a dual specificity protein kinase that belongs to the MAP kinase kinase family. This kinase is activated by mitogenic and environmental stress, and participates in the MAP kinase-mediated signaling cascade. It phosphorylates and thus activates MAPK14/p38-MAPK. This kinase can be activated by insulin, and is necessary for the expression of glucose transporter. Expression of RAS oncogene is found to result in the accumulation of the active form of this kinase, which thus leads to the constitutive activation of MAPK14, and confers oncogenic transformation of primary cells. The inhibition of this kinase is involved in the pathogenesis of Yersina pseudotuberculosis. The protein encoded by this gene is a dual specificity protein kinase that belongs to the MAP kinase kinase family. This kinase is activated by mitogenic and environmental stress, and participates in the MAP kinase-mediated signaling cascade. It phosphorylates and thus activates MAPK14/p38-MAPK. This kinase can be activated by insulin, and is necessary for the expression of glucose transporter. Expression of RAS oncogene is found to result in the accumulation of the active form of this kinase, which thus leads to the constitutive activation of MAPK14, and confers oncogenic transformation of primary cells. The inhibition of this kinase is involved in the pathogenesis of Yersina pseudotuberculosis. Multiple alternatively spliced transcript variants that encode distinct isoforms have been reported for this gene.
Molecular Weight 36 kDa
Gene ID 5606
NCBI Accession NP_002747, NM_002756
Research Area Kinases/Phosphatases
Application Notes Optimal working dilutions should be determined experimentally by the investigator.
Comment

Antigen size: 318 AA

Restrictions For Research Use only
Format Lyophilized
Reconstitution Add 50 µL of distilled water.
Concentration 1 mg/mL
Buffer PBS buffer with 2 % sucrose
Handling Advice Avoid repeated freeze-thaw cycles.
Storage -20 °C
Storage Comment For longer periods of storage, store at -20 °C
Supplier Images
anti-Mitogen-Activated Protein Kinase Kinase 3 (MAP2K3) (C-Term) antibody WB Suggested Anti-MAP2K3 Antibody Titration: 0.2-1 ug/ml
ELISA Titer: 1:312500
Positive Control: 721_B cell lysate
Background publications Prickett, Brautigan: "Cytokine activation of p38 mitogen-activated protein kinase and apoptosis is opposed by alpha-4 targeting of protein phosphatase 2A for site-specific dephosphorylation of MEK3." in: Molecular and cellular biology, Vol. 27, Issue 12, pp. 4217-27, 2007 (PubMed).

Validation Images
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