Valyl-tRNA Synthetase (VARS) (Middle Region) antibody

Details for Product No. ABIN502716
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Antigen
Synonyms
vars1, vars2, CHUNP6879, im:7137378, wu:fb07a11, wu:fb07d10, VARS, VARS2, VARSL, VARS2L, BEST:LD45671, CG4062, Dmel\\CG4062, VRS, ValRS, l(2)03531, l(2)rI255, ld45671, ECK4251, JW4215, val-act, G7A, V ... show more
vars1, vars2, CHUNP6879, im:7137378, wu:fb07a11, wu:fb07d10, VARS, VARS2, VARSL, VARS2L, BEST:LD45671, CG4062, Dmel\\CG4062, VRS, ValRS, l(2)03531, l(2)rI255, ld45671, ECK4251, JW4215, val-act, G7A, VARS1, Bat6, D17H6S56E, G7a, Vars2, TrsVal, T5E21.11, T5E21_11, TWIN 2, VALRS, VALYL TRNA SYNTHETASE show less
Epitope
Middle Region
(4), (2), (2), (2), (1), (1)
Reactivity
Mouse (Murine), Rat (Rattus), Pig (Porcine), Cow (Bovine), Dog (Canine), Human
(23), (6), (5), (1), (1), (1)
Host
Rabbit
(16), (7)
Clonality
Polyclonal
Application
Western Blotting (WB)
(20), (9), (4), (2), (1), (1)
Pubmed 1 reference available
Quantity 50 μg
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Catalog No. ABIN502716
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Immunogen Synthetic peptide directed towards the middle region of human VARS
Sequence VFDEFVDMDF GTGAVKITPA HDQNDYEVGQ RHGLEAISIM DSRGALINVP
Predicted Reactivity Bovine : 100 %, Guinea pig : 100 %, Horse : 100 %, Human : 100 %, Mouse : 100 %, Pig : 100 %, Rabbit : 100 %, Dog : 90 %, Zebrafish : 80 %
Characteristics This is a rabbit polyclonal antibody against VARS. It was validated on Western Blot using a cell lysate as a positive control.
Purification Affinity Purified
Alternative Name VARS
Background Aminoacyl-tRNA synthetases catalyze the aminoacylation of tRNA by their cognate amino acid. Because of their central role in linking amino acids with nucleotide triplets contained in tRNAs, aminoacyl-tRNA synthetases are thought to be among the first proteins that appeared in evolution. VARS belongs to class-I aminoacyl-tRNA synthetase family and is located in the class III region of the major histocompatibility complex.Aminoacyl-tRNA synthetases catalyze the aminoacylation of tRNA by their cognate amino acid. Because of their central role in linking amino acids with nucleotide triplets contained in tRNAs, aminoacyl-tRNA synthetases are thought to be among the first proteins that appeared in evolution. The protein encoded by this gene belongs to class-I aminoacyl-tRNA synthetase family and is located in the class III region of the major histocompatibility complex.
Molecular Weight 140 kDa
Gene ID 7407
NCBI Accession NP_006286, NM_006295
UniProt P26640
Application Notes Optimal working dilutions should be determined experimentally by the investigator.
Comment

Antigen size: 1264 AA

Restrictions For Research Use only
Format Lyophilized
Reconstitution Add 50 µL of distilled water.
Concentration 1 mg/mL
Buffer PBS buffer with 2 % sucrose
Handling Advice Avoid repeated freeze-thaw cycles.
Storage -20 °C
Storage Comment For longer periods of storage, store at -20 °C
Supplier Images
anti-Valyl-tRNA Synthetase (VARS) (Middle Region) antibody WB Suggested Anti-VARS Antibody Titration: 0.2-1 ug/ml
ELISA Titer: 1:312500
Positive Control: 721_B cell lysate
anti-Valyl-tRNA Synthetase (VARS) (Middle Region) antibody (2) WB Suggested Anti-VARS Antibody
Titration: 1 ug/ml
Positive Control: Wildtype Neurospora crassa
Product cited in: Ewing, Chu, Elisma et al.: "Large-scale mapping of human protein-protein interactions by mass spectrometry. ..." in: Molecular systems biology, Vol. 3, pp. 89, 2007 (PubMed).

Validation Images
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