Interleukin 1, beta (IL1B) (N-Term) antibody

Details for Product No. ABIN503515
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Antigen
Synonyms il1-b, zgc:111873, IL-1beta, Il-1b, IL1B, IL-1, IL1-BETA, IL1F2, IL-1BETA, IL1beta, IL-1B
Epitope
N-Term
(18), (17), (9), (8), (7), (6), (6), (5), (4), (4), (4), (4), (3), (3), (3), (3), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Reactivity
Human
(278), (127), (75), (29), (20), (17), (17), (10), (5), (5), (4), (3), (2), (1), (1), (1), (1)
Host
Rabbit
(259), (144), (12), (12), (10), (7), (5), (4), (3)
Clonality
Polyclonal
Conjugate
Un-conjugated
(57), (22), (12), (12), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Application
Immunohistochemistry (IHC), Western Blotting (WB)
(286), (265), (89), (60), (45), (35), (34), (33), (32), (20), (19), (14), (13), (8), (6), (5), (4), (4), (3), (2), (2), (2), (2), (2), (1), (1)
Pubmed 1 reference available
Quantity 50 μg
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Catalog No. ABIN503515
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Immunogen Synthetic peptide directed towards the N terminal of human IL1B
Sequence DLDLCPLDGG IQLRISDHHY SKGFRQAASV VVAMDKLRKM LVPCPQTFQE
Predicted Reactivity Human : 100 %
Characteristics This is a rabbit polyclonal antibody against IL1B. It was validated on Western Blot using a cell lysate as a positive control.
Purification Affinity Purified
Alternative Name IL1B
Background IL1B is a member of the interleukin 1 cytokine family. This cytokine is produced by activated macrophages as a proprotein, which is proteolytically processed to its active form by caspase 1 (CASP1/ICE). This cytokine is an important mediator of the inflammatory response, and is involved in a variety of cellular activities, including cell proliferation, differentiation, and apoptosis. The induction of cyclooxygenase-2 (PTGS2/COX2) by this cytokine in the central nervous system (CNS) is found to contribute to inflammatory pain hypersensitivity. The gene encoding IL1B and eight other interleukin 1 family genes form a cytokine gene cluster on chromosome 2.The protein encoded by this gene is a member of the interleukin 1 cytokine family. This cytokine is produced by activated macrophages as a proprotein, which is proteolytically processed to its active form by caspase 1 (CASP1/ICE). This cytokine is an important mediator of the inflammatory response, and is involved in a variety of cellular activities, including cell proliferation, differentiation, and apoptosis. The induction of cyclooxygenase-2 (PTGS2/COX2) by this cytokine in the central nervous system (CNS) is found to contribute to inflammatory pain hypersensitivity. This gene and eight other interleukin 1 family genes form a cytokine gene cluster on chromosome 2.
Molecular Weight 17 kDa
Gene ID 3553
NCBI Accession NM_000576, NP_000567
UniProt P01584
Application Notes Optimal working dilutions should be determined experimentally by the investigator.
Comment

Antigen size: 153 AA

Restrictions For Research Use only
Format Lyophilized
Reconstitution Add 50 µL of distilled water.
Concentration 1 mg/mL
Buffer PBS buffer with 2 % sucrose
Handling Advice Avoid repeated freeze-thaw cycles.
Storage -20 °C
Storage Comment For longer periods of storage, store at -20 °C
Supplier Images
anti-Interleukin 1, beta (IL1B) (N-Term) antibody WB Suggested Anti-IL1B Antibody Titration: 0.2-1 ug/ml
Positive Control: Human Placenta
anti-Interleukin 1, beta (IL1B) (N-Term) antibody (2) Sample Type: Human Macrophange Cells
Green: primary
Red: phallodin
Blue: DAPI
Yellow: green/red
Primary Dilution: 1:200
Secondary Antibody: anti-Rabbit IgG-FITC
Secondary Dilution: 1:1000
Image Submitted By: Milan Fiala
University of California, Los Angeles
Background publications Piccini, Carta, Tassi et al.: "ATP is released by monocytes stimulated with pathogen-sensing receptor ligands and induces IL-1beta and IL-18 secretion in an autocrine way." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 105, Issue 23, pp. 8067-72, 2008 (PubMed).

Validation Images
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