Proteasome (Prosome, Macropain) Subunit, beta Type, 5 (PSMB5) (Middle Region) antibody

Details for Product No. ABIN503976
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Antigen
Synonyms LMPX, MB1, X, etID309919.2, fb59c07, si:zc14a17.13, wu:fb59c07, x, zgc:86820
Epitope
Middle Region
(9), (7), (4), (2), (1), (1), (1), (1), (1), (1)
Reactivity
Mouse (Murine), Rat (Rattus), Zebrafish (Danio rerio), Cow (Bovine), Dog (Canine), Xenopus laevis, Human, Chicken
(29), (9), (4), (1)
Host
Rabbit
(26), (3), (3)
Clonality
Polyclonal
Conjugate
Un-conjugated
(1), (1), (1), (1), (1), (1)
Application
Western Blotting (WB)
(28), (16), (11), (5), (4), (4), (1), (1), (1)
Pubmed 1 reference available
Quantity 50 μg
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Catalog No. ABIN503976
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Immunogen Synthetic peptide directed towards the middle region of human PSMB5
Sequence IVAADSRATA GAYIASQTVK KVIEINPYLL GTMAGGAADC SFWERLLARQ
Predicted Reactivity Guinea pig : 100 %, Human : 100 %, Mouse : 100 %, Rabbit : 100 %, Rat : 100 %, Zebrafish : 100 %
Characteristics This is a rabbit polyclonal antibody against PSMB5. It was validated on Western Blot using a cell lysate as a positive control.
Purification Affinity Purified
Alternative Name PSMB5
Background The proteasome is a multicatalytic proteinase complex with a highly ordered ring-shaped 20S core structure. The core structure is composed of 4 rings of 28 non-identical subunits, 2 rings are composed of 7 alpha subunits and 2 rings are composed of 7 beta subunits. Proteasomes are distributed throughout eukaryotic cells at a high concentration and cleave peptides in an ATP/ubiquitin-dependent process in a non-lysosomal pathway. An essential function of a modified proteasome, the immunoproteasome, is the processing of class I MHC peptides. PSMB5 is a member of the proteasome B-type family, also known as the T1B family, that is a 20S core beta subunit in the proteasome. This catalytic subunit is not present in the immunoproteasome and is replaced by catalytic subunit 3i (proteasome beta 8 subunit).The proteasome is a multicatalytic proteinase complex with a highly ordered ring-shaped 20S core structure. The core structure is composed of 4 rings of 28 non-identical subunits, 2 rings are composed of 7 alpha subunits and 2 rings are composed of 7 beta subunits. Proteasomes are distributed throughout eukaryotic cells at a high concentration and cleave peptides in an ATP/ubiquitin-dependent process in a non-lysosomal pathway. An essential function of a modified proteasome, the immunoproteasome, is the processing of class I MHC peptides. This gene encodes a member of the proteasome B-type family, also known as the T1B family, that is a 20S core beta subunit in the proteasome. This catalytic subunit is not present in the immunoproteasome and is replaced by catalytic subunit 3i (proteasome beta 8 subunit).
Molecular Weight 28 kDa
Gene ID 5693
NCBI Accession NM_002797, NP_002788
UniProt P28074
Research Area Proteolysis / Ubiquitin, Proteasome
Application Notes Optimal working dilutions should be determined experimentally by the investigator.
Comment

Antigen size: 263 AA

Restrictions For Research Use only
Format Lyophilized
Reconstitution Add 50 µL of distilled water.
Concentration 1 mg/mL
Buffer PBS buffer with 2 % sucrose
Handling Advice Avoid repeated freeze-thaw cycles.
Storage -20 °C
Storage Comment For longer periods of storage, store at -20 °C
Supplier Images
anti-Proteasome (Prosome, Macropain) Subunit, beta Type, 5 (PSMB5) (Middle Region) antibody WB Suggested Anti-PSMB5 Antibody Titration: 0.2-1 ug/ml
ELISA Titer: 1:62500
Positive Control: MCF7 cell lysate
Product cited in: Ewing, Chu, Elisma et al.: "Large-scale mapping of human protein-protein interactions by mass spectrometry. ..." in: Molecular systems biology, Vol. 3, pp. 89, 2007 (PubMed).

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