PSMB5 antibody (C-Term)

Catalog No. ABIN6264477

Product Details anti-PSMB5 Antibody

Target: PSMB5 (Proteasome (Prosome, Macropain) Subunit, beta Type, 5 (PSMB5))

Binding Specificity: C-Term

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This PSMB5 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), ELISA

Specificity: PSMB5 Antibody detects endogenous levels of total PSMB5.

Predicted Reactivity: Bovine,Horse,Sheep,Rabbit,Dog,Xenopus

Purification: The antiserum was purified by peptide affinity chromatography using SulfoLink^TM Coupling Resin (Thermo Fisher Scientific).

Immunogen: A synthesized peptide derived from human PSMB5, corresponding to a region within C-terminal amino acids.

Isotype: IgG

Application Details

Application Notes: WB 1:500-1:2000, IHC 1:50-1:200, ELISA(peptide) 1:20000-1:40000

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: Rabbit IgG in phosphate buffered saline , pH 7.4, 150 mM NaCl, 0.02 % sodium azide and 50 % glycerol.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Store at -20 °C. Stable for 12 months from date of receipt.

Expiry Date: 12 months

Target Details for PSMB5

Target: PSMB5 (Proteasome (Prosome, Macropain) Subunit, beta Type, 5 (PSMB5))

Alternative Name: PSMB5 (PSMB5 Products)

Synonyms: LMPX antibody, MB1 antibody, X antibody, etID309919.2 antibody, fb59c07 antibody, si:zc14a17.13 antibody, wu:fb59c07 antibody, x antibody, zgc:86820 antibody, proteasome subunit beta 5 antibody, proteasome (prosome, macropain) subunit, beta type 5 antibody, proteasome subunit beta 5 L homeolog antibody, proteasome (prosome, macropain) subunit, beta type, 5 antibody, PSMB5 antibody, psmb5 antibody, Psmb5 antibody, psmb5.L antibody

Background:

Description: Component of the 20S core proteasome complex involved in the proteolytic degradation of most intracellular proteins. This complex plays numerous essential roles within the cell by associating with different regulatory particles. Associated with two 19S regulatory particles, forms the 26S proteasome and thus participates in the ATP-dependent degradation of ubiquitinated proteins. The 26S proteasome plays a key role in the maintenance of protein homeostasis by removing misfolded or damaged proteins that could impair cellular functions, and by removing proteins whose functions are no longer required. Associated with the PA200 or PA28, the 20S proteasome mediates ubiquitin-independent protein degradation. This type of proteolysis is required in several pathways including spermatogenesis (20S-PA200 complex) or generation of a subset of MHC class I-presented antigenic peptides (20S-PA28 complex). Within the 20S core complex, PSMB5 displays a chymotrypsin-like activity.

Gene: PSMB5

Molecular Weight: 28kDa

Gene ID: 5693

UniProt: P28074

Pathways: Mitotic G1-G1/S Phases, DNA Replication, Synthesis of DNA