AIF antibody (Middle Region)

Catalog No. ABIN504574

This Rabbit Polyclonal antibody specifically detects AIF in WB. It exhibits reactivity toward Human, Rat, Mouse, Pig, Cow, Dog, Horse and Rabbit.

Quick Overview for AIF antibody (Middle Region) (ABIN504574)

Target: AIF (AIFM1) (Apoptosis-Inducing Factor, Mitochondrion-Associated, 1 (AIFM1))

Reactivity: Human, Rat, Mouse, Pig, Cow, Dog, Horse, Rabbit

Host: Rabbit

Clonality: Polyclonal

Conjugate: This AIF antibody is un-conjugated

Application: Western Blotting (WB)

Product Details anti-AIF Antibody

Binding Specificity: Middle Region

Sequence: VIFYLRDKVV VGIVLWNIFN RMPIARKIIK DGEQHEDLNE VAKLFNIHED

Predicted Reactivity: Cow: 100%, Dog: 100%, Horse: 100%, Human: 100%, Mouse: 100%, Pig: 100%, Rabbit: 100%, Rat: 100%

Characteristics: This is a rabbit polyclonal antibody against AIFM1. It was validated on Western Blot using a cell lysate as a positive control.

Purification: Affinity Purified

Immunogen: The immunogen is a synthetic peptide directed towards the middle region of human AIFM1

Application Details

Application Notes: Optimal working dilutions should be determined experimentally by the investigator.

Comment:

Antigen size: 613 AA

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: Liquid. Purified antibody supplied in 1x PBS buffer with 0.09 % (w/v) sodium azide and 2 % sucrose.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freeze-thaw cycles.

Storage: -20 °C

Storage Comment: For short term use, store at 2-8°C up to 1 week. For long term storage, store at -20°C in small aliquots to prevent freeze-thaw cycles.

Target Details for AIF

Target: AIF (AIFM1) (Apoptosis-Inducing Factor, Mitochondrion-Associated, 1 (AIFM1))

Alternative Name: AIFM1

Background: AIFM1 is a flavoprotein essential for nuclear disassembly in apoptotic cells that is found in the mitochondrial intermembrane space in healthy cells. Induction of apoptosis results in the translocation of this protein to the nucleus where it effects chromosome condensation and fragmentation. In addition, this protein induces mitochondria to release the apoptogenic proteins cytochrome c and caspase-9. Several alternative transcripts encoding different isoforms have been identified for this gene.This gene encodes a flavoprotein essential for nuclear disassembly in apoptotic cells that is found in the mitochondrial intermembrane space in healthy cells. Induction of apoptosis results in the translocation of this protein to the nucleus where it effects chromosome condensation and fragmentation. In addition, this gene product induces mitochondria to release the apoptogenic proteins cytochrome c and caspase-9. Three alternative transcripts encoding different isoforms have been identified for this gene.
Alias Symbols: AIF, MGC111425, PDCD8, COXPD6
Protein Interaction Partner: ISG15, STAU1, BAG6, UBC, NEDD8, MDM2, ASB12, RNF2, BMI1, SUZ12, PARK2, BAG3, ADRB2, HDAC11, OXSR1, ILK, PAN2, NOS2, MLH1, CFTR, ESR1, TST, FSCN1, CPOX, SHC1, FBXO6, TSC22D4, CAND1, COPS5, CUL1, CUL2, CUL3, CUL5, TONSL, BLNK, PIDD1, XIAP, AMFR, Tubb4b, Tub
Protein Size: 613

Molecular Weight: 67 kDa

Gene ID: 9131

NCBI Accession: NM_004208, NP_004199

UniProt: O95831

Pathways: Apoptosis, Positive Regulation of Endopeptidase Activity, Cell RedoxHomeostasis, Smooth Muscle Cell Migration, Warburg Effect