GFP antibody
| Antigen |
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| Clonality |
Monoclonal (GF28R) |
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Host
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Application
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 Alternatives Dot Blot (Dot), ELISA, Immunoprecipitation (IP), Immunocytochemistry (ICC), Western Blotting (WB)
Western Blotting (WB) (164), ELISA (85), Immunofluorescence (IF) (83), Flow Cytometry (FACS) (60), Immunoprecipitation (IP) (59), Immunohistochemistry (Frozen Sections) (IHC (fro)) (33), Enzyme Immunoassay (EIA) (31), Immunohistochemistry (IHC) (26), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) (18), Immunocytochemistry (ICC) (10), Immunohistochemistry (Formalin-fixed Sections) (IHC (f)) (6), Immunoelectron Microscopy (IEM) (3), Dot Blot (Dot) (2), To be determined by user (TBD) (2)
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| Catalog no. |
ABIN559689 |
| Quantity |
100ug (1mg/mL) |
| Price |
199.00 $ Plus shipping costs $35.00
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| Shipping to |
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| Availability |
Ships within 5 to 7 Business Days |
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Immunogen
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GFP from the jellyfish Aequorea Victoria N-terminal peptide-KLH conjugates
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Isotype
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IgG1 (Matching secondary antibodies)
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Clone
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GF28R
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Specificity
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Recognizes native and denatured forms of GFP and its variants such as: EGFP, YFP, EYFP, and CFP
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Sensitivity
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WB (with ECL): 1-10ng of purified GFP or GFP fusion proteins
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Application Notes
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WB (with ECL): 1:1000-3000 dilution (incubate for one hour at room temperature) / IS: 1:500-2000 dilution / For best results with other assays (e.g.: Dot, ELISA, IP, etc), please determine optimal working dilution by titration test
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Concentration
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1mg/mL
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Purification
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Protein A affinity chromatography from mouse ascites fluid
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Buffer
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10mM PBS, pH 7.2, 0.05% NaN3
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Storage
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Store at -20°C for up to 2 years or more. Centrifuge after first thaw to maximize product recovery. Avoid repeated freeze-thaw cycles by aliquots. Store aliquots at 4°C for several days to weeks
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Restrictions
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For Research Use only
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Alternatives for antigen "GFP", type "Antibodies"
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Hosts
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Rabbit (115), Mouse (75), Goat (35), Chicken (14), Sheep (2)
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Reactivities
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Aequorea victoria (79), Human (23), Mouse (Murine) (15), Fusion Protein (Tag) (14), Rat (Rattus) (12), Cow (Bovine) (5), Dog (Canine) (5), Rabbit (4), All Species (3), Chicken (3), Jellyfish (1)
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Applications
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Western Blotting (WB) (164), ELISA (85), Immunofluorescence (IF) (83), Flow Cytometry (FACS) (60), Immunoprecipitation (IP) (59), Immunohistochemistry (Frozen Sections) (IHC (fro)) (33), Enzyme Immunoassay (EIA) (31), Immunohistochemistry (IHC) (26), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) (18), Immunocytochemistry (ICC) (10), Immunohistochemistry (Formalin-fixed Sections) (IHC (f)) (6), Immunoelectron Microscopy (IEM) (3), Dot Blot (Dot) (2), To be determined by user (TBD) (2)
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Conjugates
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HRP (17), Biotin (15), FITC (10), Alkaline Phosphatase (AP) (4), Texas Red (TR) (4), Alexa Flour 488 (3), Alexa Fluor 350 (3), Alexa Fluor 488 (3), Alexa Fluor 555 (3), Alexa Fluor 647 (3), Cy3 (3), Cy5 (3), Cy5.5 (3), Cy7 (3), Gold (3), PE (3), PE,Cy3 (3), PE,Cy5 (3), PE,Cy5.5 (3), PE,Cy7 (3), RBITC (3), Rhodamine (1), TRITC (1)
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Epitopes
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N-Term (4)
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