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Checkpoint with Forkhead and Ring Finger Domains, E3 Ubiquitin Protein Ligase (CHFR) (AA 1-652) antibody

Details for Product No. ABIN565913
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Antigen
Synonyms 5730484M20Rik, C230082M18, RNF116, RNF196, fc43f10, si:dkey-69h6.7, wu:fc43f10
Epitope
AA 1-652
(10), (7), (7), (2), (2), (2), (2), (1), (1), (1), (1)
Reactivity
Human
(49), (20), (20), (1), (1), (1), (1)
Host
Mouse
(34), (11), (5)
Clonality (Clone)
Monoclonal ()
Conjugate
Un-conjugated
(3), (3), (3), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1)
Application
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB), ELISA, RNA Interference (RNAi)
(36), (24), (13), (10), (8)
Pubmed 7 references available
Quantity 100 µg
Shipping to United States (Change)
Availability Will be delivered in 11 to 12 Business Days
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Catalog No. ABIN565913
394.24 $
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Immunogen CHFR (AAH12072.1, 1 a.a. ~ 652 a.a) full-length recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.
Immunogen sequence: MERPEEGKQSPPPQPWGRLL RLGAEEGEPHVLLRKREWTI GRRRGCDLSFPSNKLVSGDH CRIVVDEKSGQVTLEDTSTS GTVINKLKVVKKQTCPLQTG DVIYLVYRKNEPEHNVAYLY ESLSEKQGMTQESFDTSGAG AGRGADPRVPPSSPATQVCF EEPQPSTSTSDLFPTASASS TEPSPAGRERSSSCGSGGGG ISPKGSGPSVASDEVSSFAS ALPDRKTASFSSLEPQDQED LEPVKKKMRGDGDLDLNGQL LVAQPRRNAQTVHEDVRAAA GKPDKMEETLTCIICQDLLH DCVSLQPCMHTFCAACYSGW MERSSLCPTCRCPVERICKN HILNNLVEAYLIQHPDKSRS EEDVQSMDARNKITQDMLQP KVRRSFSDEEGSSEDLLELS DVDSESSDISQPYVVCRQCP EYRRQAAQPPHCPAPEGEPG APQALGDAPSTSVSLTTAVQ DYVCPLQGSHALCTCCFQPM PDRRVEREQDPRVAPQQCAV CLQPFCHLYWGCTRTGCYGC LAPFCELNLGDKCLDGVLNN NSYESDILKNYLATRGLTWK NMLTESLVALQRGVFLLSDY RVTGDTVLCYCCGLRSFREL TYQYRQNIPASELPVAVTSR PDCYWGRNCRTQVKAHHAMK FNHICEQTRFKN
Clone 1H3-A12
Isotype IgG1 kappa
Characteristics Mouse monoclonal antibody raised against a full length recombinant CHFR.
Alternative Name CHFR / RNF196
Background Gene: checkpoint with forkhead and ring finger domains
Synonyms: FLJ10796, FLJ33629, RNF116, RNF196
Gene ID 55743, BC012072
Research Area Cancer, Cell Cycle
Application Notes Application specialization: Recombinant protein
Restrictions For Research Use only
Buffer 1x PBS, pH 7.2
Storage -20 °C
Storage Comment Store at -20 °C or lower. Aliquot to avoid repeated freezing and thawing.
Supplier Images
anti-Checkpoint with Forkhead and Ring Finger Domains, E3 Ubiquitin Protein Ligase (CHFR) (AA 1-652) antibody Immunoperoxidase of monoclonal antibody to CHFR on formalin-fixed paraffin-embedded human small Intestine. [antibody concentration 2 µg/mL]
anti-Checkpoint with Forkhead and Ring Finger Domains, E3 Ubiquitin Protein Ligase (CHFR) (AA 1-652) antibody (2) Western blot analysis of CHFR over-expressed 293 cell line, cotransfected with CHFR Validated Chimera RNAi (Lane 2) or non-transfected control (Lane 1). Blot probed with CHFR monoclonal antibody (M01), clone 1H3-A12 (ABIN565913). GAPDH (36.1 kDa) used as specificity and loading control.
anti-Checkpoint with Forkhead and Ring Finger Domains, E3 Ubiquitin Protein Ligase (CHFR) (AA 1-652) antibody (3) Detection limit for recombinant GST tagged CHFR is approximately 0.1 ng/mL as a capture antibody.
anti-Checkpoint with Forkhead and Ring Finger Domains, E3 Ubiquitin Protein Ligase (CHFR) (AA 1-652) antibody (4) Western Blot detection against Immunogen (97.46 KDa) .
Product cited in: Milne, Sitarz, Carvalho et al.: "Molecular analysis of primary gastric cancer, corresponding xenografts, and 2 novel gastric carcinoma cell lines reveals novel alterations in gastric carcinogenesis." in: Human pathology, Vol. 38, Issue 6, pp. 903-13, 2007 (PubMed).

Privette, González, Ding et al.: "Altered expression of the early mitotic checkpoint protein, CHFR, in breast cancers: implications for tumor suppression." in: Cancer research, Vol. 67, Issue 13, pp. 6064-74, 2007 (PubMed).

Privette, Weier, Nguyen et al.: "Loss of CHFR in human mammary epithelial cells causes genomic instability by disrupting the mitotic spindle assembly checkpoint." in: Neoplasia (New York, N.Y.), Vol. 10, Issue 7, pp. 643-52, 2008 (PubMed).

Gao, Xin, Zhang et al.: "Mechanism and pathobiologic implications of CHFR promoter methylation in gastric carcinoma." in: World journal of gastroenterology : WJG, Vol. 14, Issue 32, pp. 5000-7, 2008 (PubMed).

Hu, Huang, Sun et al.: "Pathobiologic implications of methylation and expression status of Runx3 and CHFR genes in gastric cancer." in: Medical oncology (Northwood, London, England), Vol. 28, Issue 2, pp. 447-54, 2011 (PubMed).

Fujita, Yamamoto, Imamura et al.: "Expression level of the mitotic checkpoint protein and G2-M cell cycle regulators and prognosis in gastrointestinal stromal tumors in the stomach." in: Virchows Archiv : an international journal of pathology, Vol. 460, Issue 2, pp. 163-9, 2012 (PubMed).

Kashima, Idogawa, Mita et al.: "CHFR protein regulates mitotic checkpoint by targeting PARP-1 protein for ubiquitination and degradation." in: The Journal of biological chemistry, Vol. 287, Issue 16, pp. 12975-84, 2012 (PubMed).

Validation Images
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