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CHFR antibody (Checkpoint with Forkhead and Ring Finger Domains) (C-Term) Primary Antibody

Verified CHFR Reactivity: Human ELISA, IHC Host: Goat Polyclonal unconjugated
Catalog No. ABIN184918
$481.84
Plus shipping costs $45.00
100 μg
local_shipping Shipping to: United States
Delivery in 6 to 7 Business Days
  • Target
    CHFR
    Binding Specificity
    • 14
    • 7
    • 7
    • 4
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    C-Term
    Reactivity
    • 50
    • 14
    • 13
    • 2
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    Human
    Host
    • 40
    • 6
    • 4
    Goat
    Clonality
    • 47
    • 3
    Polyclonal
    Conjugate
    • 15
    • 6
    • 4
    • 4
    • 3
    • 3
    • 3
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    This CHFR antibody is un-conjugated
    Application
    • 34
    • 29
    • 13
    • 3
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    ELISA, Immunohistochemistry (IHC)
    Purpose
    CHFR
    Sequence
    KFNHICEQTR FKN
    Specificity
    This antibody is expected to recognise all reported isoforms.
    Predicted Reactivity
    Human, Mouse, Rat, Pig Cow
    Purification
    Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
    Grade
    Verified
    Immunogen
    Peptide with sequence KFNHICEQTRFKN, from the C Terminus of the protein sequence according to NP_001154816.1, NP_001154817.1, NP_001154818.1, NP_060693.2, NP_001154819.1.
    Isotype
    IgG
  • Application Notes

    DS WB Results: Preliminary experiments gave an approx 80 kDa band in human brain lysates at 0.5 μg/mL, this band was successfully blocked by incubation with the immunising peptide. Please note that currently we cannot find an explanation in the literature for the band we observe given the predicted size of approx. 72kDda according to NP_060693.

    DS IHC Results: In paraffin embedded Human Brain (Cerebellum) shows stained Purkinje cells. Recommended concentration: 2.5 μg/mL.

    Peptide ELISA: antibody detection limit dilution 1:64000.

    Restrictions
    For Research Use only
  • Format
    Liquid
    Concentration
    0.5 mg/mL
    Buffer
    Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.
    Preservative
    Sodium azide
    Precaution of Use
    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
    Handling Advice
    Minimize freezing and thawing.
    Storage
    -20 °C
    Storage Comment
    Aliquot and store at -20°C, with minimal freeze/thawing. A working aliquot may be refrigerated at 4°C for a few weeks and still remain viable.
  • Target
    CHFR
    Alternative Name
    CHFR (CHFR Antibody Abstract)
    Synonyms
    RNF116, RNF196, CHFR, rnf116, rnf196, 5730484M20Rik, C230082M18, checkpoint with forkhead and ring finger domains, checkpoint with forkhead and ring finger domains, E3 ubiquitin protein ligase, checkpoint with forkhead and ring finger domains, E3 ubiquitin protein ligase L homeolog, CHFR, chfr, Chfr, chfr.L
    Background
    CHFR, checkpoint with forkhead and ring finger domains, FLJ10796, FLJ33629, RNF116, RNF196, E3 ubiquitin-protein ligase CHFR, RING finger protein 196
    Gene ID
    55743
    NCBI Accession
    NP_001154816, NP_001154817, NP_001154818, NP_060693, NP_001154819
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