Isocitrate Dehydrogenase antibody (Internal Region)

Catalog No. ABIN569649

The Goat Polyclonal anti-Isocitrate Dehydrogenase antibody (ABIN569649) specifically detects Isocitrate Dehydrogenase in WB and EIA. The antibody is reactive with Human, Rat, Mouse, Dog, Sheep and Cow samples.

Quick Overview for Isocitrate Dehydrogenase antibody (Internal Region) (ABIN569649)

Target: Isocitrate Dehydrogenase (IDH)

Reactivity: Human, Rat, Mouse, Dog, Sheep, Cow

Host: Goat

Clonality: Polyclonal

Conjugate: This Isocitrate Dehydrogenase antibody is un-conjugated

Application: Western Blotting (WB), Enzyme Immunoassay (EIA)

Product Details anti-Isocitrate Dehydrogenase Antibody

Binding Specificity: AA 65-77, Internal Region

Sequence: KKHNVGVKCA TIT

Specificity: Recognizes IDH1 (65-77).

Cross-Reactivity (Details): Species reactivity (expected):Canine (Dog), Sheep.
Species reactivity (tested):Human, Mouse, Rat.

Purification: Ammonium Sulphate Precipitation followed by antigen Affinity Chromatography using the immunizing peptide

Immunogen: Peptide with sequence from the internal region of the protein sequence according to NP_005887.2.

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Restrictions: For Research Use only

Handling

Concentration: 0.5 mg/mL

Buffer: Tris saline, pH ~7.3 with 0.02 % Sodium Azide and 0.5 % BSA

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C/-20 °C

Storage Comment: Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.

Target Details for Isocitrate Dehydrogenase

Target: Isocitrate Dehydrogenase (IDH)

Alternative Name: Isocitrate Dehydrogenase / IDH

Background: IDH1 belongs to two distinct subclasses. The protein is the NADP(+)-dependent isocitrate dehydrogenase found in the cytoplasm and peroxisomes. This protein contains the PTS-1 peroxisomal targeting signal sequence. The presence of this enzyme in peroxisomes suggests roles in the regeneration of NADPH for intraperoxisomal reductions, such as the conversion of 2, 4-dienoyl-CoAs to 3-enoyl-CoAs, as well as in peroxisomal reactions that consume 2-oxoglutarate, namely the alpha-hydroxylation of phytanic acid. The cytoplasmic enzyme serves a significant role in cytoplasmic NADPH production.Synonyms: Cytosolic NADP-isocitrate dehydrogenase, ICDH, IDP, NADP(+)-specific ICDH, Oxalosuccinate decarboxylase, PICD, cytoplasmic Isocitrate dehydrogenase [NADP]

Molecular Weight: 46.7kDa (NP_005887.2).

Gene ID: 3417

NCBI Accession: NP_005887

UniProt: O75874