IREB2 antibody (AA 489-503)

Catalog No. ABIN574322

Product Details anti-IREB2 Antibody

Target: IREB2 (Iron-Responsive Element Binding Protein 2 (IREB2))

Binding Specificity: AA 489-503

Reactivity: Human, Pig, Monkey

Host: Goat

Clonality: Polyclonal

Conjugate: This IREB2 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Brand: IHC-plus™

Specificity: Human IREB2.

Predicted Reactivity: Percent identity by BLAST analysis: Human, Gorilla, Orangutan, Gibbon, Monkey, Marmoset, Panda, Pig (100%) Elephant, Horse, Rabbit (93%) Bovine (87%) Mouse, Rat, Dog, Opossum, Platypus (80%).

Purification: Immunoaffinity purified

Immunogen: Synthetic peptide C-SIHYEGSEYKLSHGS from an internal region of human IREB2 (NP_004127.1). Percent identity by BLAST analysis: Human, Gorilla, Orangutan, Gibbon, Monkey, Marmoset, Panda, Pig (100%), Elephant, Horse, Rabbit (93%), Bovine (87%), Mouse, Rat, Dog, Opossum, Platypus (80%).

Type of Immunogen: Synthetic peptide

Application Details

Application Notes: Approved: IHC, IHC-P (3.75 μg/mL), Peptide-ELISA (1:32000), WB (1 - 3 μg/mL)

Usage: Western Blot: Approx 110kD band observed in Human Liver lysates (calculated MW of 105kD according to NP_004127.1). Recommended concentration: 1-3 μg/mL. An additional band of unknown identity was also consistently observed at 60kD. This band was successfully blocked by incubation with the immunizing peptide. Have any further splice variants/modified forms been reported?

Comment:

Target Species of Antibody: Human

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: Tris-buffered saline,  pH 7.3, 0.5 % BSA, 0.02 % sodium azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Minimize freezing and thawing.

Storage: -20 °C

Storage Comment: Store at -20°C. Avoid freeze-thaw cycles.

Target Details for IREB2

Target: IREB2 (Iron-Responsive Element Binding Protein 2 (IREB2))

Alternative Name: IREB2 / IRP2 (IREB2 Products)

Synonyms: IREB2 antibody, im:7153062 antibody, DKFZp468N047 antibody, D9Ertd85e antibody, Irp2 antibody, ACO3 antibody, IRP2 antibody, IRP2AD antibody, IREBP2 antibody, iron responsive element binding protein 2 antibody, iron-responsive element binding protein 2 antibody, iron responsive element binding protein 2 L homeolog antibody, IREB2 antibody, ireb2 antibody, Ireb2 antibody, ireb2.L antibody

Background: Name/Gene ID: IREB2

Synonyms: IREB2, ACO3, Iron regulatory protein 2, IRP2AD, IRE-BP 2, IRP2

Gene ID: 3658

NCBI Accession: NP_004127

UniProt: P48200

Pathways: Transition Metal Ion Homeostasis