AKT1 antibody (pSer473)

Catalog No. ABIN600416

The Rabbit Polyclonal anti-AKT1 antibody is suitable to detect AKT1 in samples from Human, Mouse, Rat, Chicken, Cow, Dog, Pig, Zebrafish (Danio rerio), Monkey, Sheep, Xenopus laevis and Horse. It has been validated for WB and IHC.

Quick Overview for AKT1 antibody (pSer473) (ABIN600416)

Target: AKT1 (V-Akt Murine Thymoma Viral Oncogene Homolog 1 (AKT1))

Reactivity: Human, Mouse, Rat, Chicken, Cow, Dog, Pig, Zebrafish (Danio rerio), Monkey, Sheep, Xenopus laevis, Horse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This AKT1 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC)

Product Details anti-AKT1 Antibody

Binding Specificity: pSer473

Specificity: Recognizes human Akt1 when phosphorylated at serine 473. Species cross-reactivity: mouse, rat.

Purification: Immunoaffinity purified

Immunogen: Synthetic phosphopeptide corresponding to human Akt around the phosphorylation site of serine 473 (Q-F-SP-Y-S).

Type of Immunogen: Synthetic phospho-peptide

Isotype: IgG

Application Details

Application Notes: Approved: IHC (1:50 - 1:100), WB (1:500 - 1:1000)

Usage: Suitable for use in Western Blot and Immunohistochemistry. Western Blot: 1:500-1:1000. Immunohistochemistry: 1:50-1:100.

Comment:

Target Species of Antibody: Human

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: PBS (without Mg2+, Ca2+),  pH 7.4, 150 mM sodium chloride, 0.02 % sodium azide, 50 % glycerol

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Aliquot to Avoid repeated freezing and thawing.

Storage: 4 °C,-20 °C

Storage Comment: May be stored at 4°C for short-term only. Aliquot to avoid freeze-thaw cycles. Store at -20°C. Aliquots are stable for at least 1 year.

Target Details for AKT1

Target: AKT1 (V-Akt Murine Thymoma Viral Oncogene Homolog 1 (AKT1))

Alternative Name: AKT1 / AKT

Background: Name/Gene ID: AKT1
Subfamily: RAC/AKT
Family: Protein Kinase

Synonyms: AKT1, AKT, AKT-1, C-AKT, Pan-AKT, PKB alpha, PKBalpha, Proto-oncogene c-Akt, Protein kinase B, Rac protein kinase alpha, PRKBA, Protein kinase B alpha, RAC-PK-alpha, PKB, PKB-ALPHA, RAC, RAC-ALPHA

Gene ID: 207

UniProt: P31749

Pathways: PI3K-Akt Signaling, RTK Signaling, TCR Signaling, AMPK Signaling, Interferon-gamma Pathway, TLR Signaling, Fc-epsilon Receptor Signaling Pathway, EGFR Signaling Pathway, Neurotrophin Signaling Pathway, Response to Water Deprivation, Regulation of Actin Filament Polymerization, Carbohydrate Homeostasis, Glycosaminoglycan Metabolic Process, Cellular Glucan Metabolic Process, Regulation of Muscle Cell Differentiation, Cell-Cell Junction Organization, Regulation of Cell Size, Skeletal Muscle Fiber Development, Regulation of Carbohydrate Metabolic Process, Hepatitis C, Protein targeting to Nucleus, CXCR4-mediated Signaling Events, Signaling Events mediated by VEGFR1 and VEGFR2, Negative Regulation of intrinsic apoptotic Signaling, Thromboxane A2 Receptor Signaling, Signaling of Hepatocyte Growth Factor Receptor, Positive Regulation of fat Cell Differentiation, VEGFR1 Specific Signals, VEGF Signaling, Warburg Effect