V5 Epitope Tag antibody (Biotin)

Catalog No. ABIN602522

Product Details anti-V5 Epitope Tag Antibody (Biotin)

Target: V5 Epitope Tag

Reactivity: Please inquire

Host: Mouse

Clonality: Monoclonal

Conjugate: This V5 Epitope Tag antibody is conjugated to Biotin

Application: Western Blotting (WB), ELISA, Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunohistochemistry (IHC)

Sequence: GKPIPNPLLG LDST

Specificity: Recognizes a small epitope, termed Pk, present on the P/V proteins of the paramyxovirus, SV5. Has been used to detect recombinant proteins, some of which include transmembrane and secreted proteins, which have been tagged with this epitope. Usually, a 14 amino acid tag has been added to the recombinant proteins, although a smaller epitope of 9 amino acids (that as a peptide inhibit the binding of the monoclonal antibody to its native protein) has also been successfully used. The 14 amino acid epitope is, gly lys pro ile pro asn pro leu leu gly leu asp ser thr. (The 9 amino acid epitope is underlined.

Purification: Protein G purified

Immunogen: Paramyxovirus SV5, Simian-Virus 5.

Type of Immunogen: Virus

Isotype: IgG2a

Application Details

Application Notes: Approved: ELISA, IHC, IHC-Fr, WB (1:1000 - 1:5000)

Usage: Western Blot:1:1000-1:5000. ELISA. Immunohistochemistry (frozen). The applications listed have been tested for the unconjugated form of this product. Other forms have not been tested.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: IgG conjugated to Biotin. Supplied as a liquid, 0.1 % sodium azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Aliquot to Avoid repeated freezing and thawing.

Storage: 4 °C,-20 °C

Storage Comment: May be stored at 4°C for short-term only. Aliquot to avoid freeze-thaw cycles. Store at -20°C. Aliquots are stable for at least 1 year.

Target Details for V5 Epitope Tag

Target: V5 Epitope Tag

Alternative Name: V5 Tag (V5 Epitope Tag Products)

Target Type: Tag