E-cadherin antibody (AA 381-481)

Catalog No. ABIN614587

This Mouse Monoclonal antibody specifically detects E-cadherin in WB, IHC (p) and EIA. It exhibits reactivity toward Human.It has been mentioned in 1 publication

Quick Overview for E-cadherin antibody (AA 381-481) (ABIN614587)

Target: E-cadherin (CDH1) (Cadherin 1, Type 1, E-Cadherin (Epithelial) (CDH1))

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This E-cadherin antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Enzyme Immunoassay (EIA)

Clone: 3F4

Product Details anti-E-cadherin Antibody

Binding Specificity: AA 381-481

Specificity: This antibody reacts to Cadherin 1, Type 1, E-cadherin (epithelial) (CDH1).

Purification: Protein A Chromatography

Immunogen: Recombinant protein, CDH1 (NP_004351, 381 a.a. ~ 481 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. Genename: CDH1

Isotype: IgG1

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Restrictions: For Research Use only

Handling

Buffer: PBS, pH 7.2

Handling Advice: Avoid repeated freezing and thawing.

Storage: -20 °C

References for anti-E-cadherin antibody (ABIN614587)

Gauster, Maninger, Siwetz, Deutsch, El-Heliebi, Kolb-Lenz, Hiden, Desoye, Herse, Prokesch: "Downregulation of p53 drives autophagy during human trophoblast differentiation." in: Cellular and molecular life sciences : CMLS, Vol. 75, Issue 10, pp. 1839-1855, (2018) (PubMed).

Target Details for E-cadherin

Target: E-cadherin (CDH1) (Cadherin 1, Type 1, E-Cadherin (Epithelial) (CDH1))

Alternative Name: CD324 / Cadherin-1

Background: E Cadherin (CDH1) is a classical cadherin from the cadherin superfamily. It is a calcium dependent cell-cell adhesion glycoprotein composed of five extracellular cadherin repeats, a transmembrane region and a highly conserved cytoplasmic tail. Mutations in this gene are correlated with gastric, breast, colorectal, thyroid and ovarian cancer. Loss of function is thought to contribute to progression in cancer by increasing proliferation, invasion, and/or metastasis. The ectodomain of this protein mediates bacterial adhesion to mammalian cells and the cytoplasmic domain is required for internalization. Identified transcript variants arise from mutation at consensus splice sites.Synonyms: CAM 120/80, CDH1, CDHE, E-cadherin, Epithelial cadherin, UVO, Uvomorulin

Gene ID: 999

NCBI Accession: NP_004351

UniProt: P12830

Pathways: WNT Signaling, Sensory Perception of Sound, Cell-Cell Junction Organization, Tube Formation