E-cadherin antibody

Catalog No. ABIN614765

There are 3+ publications for this product available. The Mouse Monoclonal anti-E-cadherin antibody is suitable to detect E-cadherin in samples from Human. It has been validated for WB, FACS, IF, IP and IHC (fro).

Quick Overview for E-cadherin antibody (ABIN614765)

Target: E-cadherin (CDH1) (Cadherin 1, Type 1, E-Cadherin (Epithelial) (CDH1))

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This E-cadherin antibody is un-conjugated

Application: Western Blotting (WB), Flow Cytometry (FACS), Immunofluorescence (IF), Immunoprecipitation (IP), Immunohistochemistry (Frozen Sections) (IHC (fro))

Clone: 67A4

Product Details anti-E-cadherin Antibody

Specificity: This antibody recognizes CD324 / E-cadherin, an approximately 100 kDa epithelial cell adhesion molecule, whose detection is important for determination of invasive potential of epithelial neoplasms.

Cross-Reactivity (Details): Species reactivity (tested):Human

Purification: Protein-A affinity chromatography

Purity: > 95 % pure by SDS-PAGE

Immunogen: T-47D cells

Isotype: IgG1

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Restrictions: For Research Use only

Handling

Concentration: 1.0 mg/mL

Buffer: Phosphate buffered saline (PBS) with 15 mM sodium azide, approx. pH 7.4

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C/-20 °C

Storage Comment: Store at 2 - 8 °C for up to one month or (in aliquots) at -20 °C for longer.

References for anti-E-cadherin antibody (ABIN614765)

Lin, Liao, Lee, Hung, Sayion, Chen, Kang, Huang, Cherng: "Molecular events associated with epithelial to mesenchymal transition of nasopharyngeal carcinoma cells in the absence of Epstein-Barr virus genome." in: Journal of biomedical science, Vol. 16, pp. 105, (2009) (PubMed).

Furio, Guezennec, Ducarre, Guesnet, Peguet-Navarro: "Differential effects of allergens and irritants on early differentiating monocyte-derived dendritic cells." in: European journal of dermatology : EJD, Vol. 18, Issue 2, pp. 141-7, (2008) (PubMed).

Caberg, Hubert, Begon, Herfs, Roncarati, Boniver, Delvenne: "Silencing of E7 oncogene restores functional E-cadherin expression in human papillomavirus 16-transformed keratinocytes." in: Carcinogenesis, Vol. 29, Issue 7, pp. 1441-7, (2008) (PubMed).

Target Details for E-cadherin

Target: E-cadherin (CDH1) (Cadherin 1, Type 1, E-Cadherin (Epithelial) (CDH1))

Alternative Name: CD324 / Cadherin-1

Background: CD324 / E-cadherin is an epithelial cell surface molecule, which provides calcium-dependent homophilic interactions with E-cadherin of another cell. These intaractions take part in morphogenetic programs controlling the maintenance of the structural and functional integrity of epithelia and affect invasive potential of epithelial neoplasms. CD324 / E-cadherin is implicated in cell growth and differentiation, cell recognition, and sorting during developmental morphogenesis, as well as in aggregation-dependent cell survival. CD324 / E-cadherin-mediated cell adhesion system is highly regulated from inside the cell by a number of intracellular signaling pathways.Synonyms: CAM 120/80, CDH1, CDHE, E-cadherin, Epithelial cadherin, UVO, Uvomorulin

Gene ID: 999, 9606

UniProt: P12830

Pathways: WNT Signaling, Sensory Perception of Sound, Cell-Cell Junction Organization, Tube Formation