SIRT1 antibody (Internal Region)

Catalog No. ABIN615980

The Goat Polyclonal anti-SIRT1 antibody is suitable to detect SIRT1 in samples from Human and Mouse. It has been validated for WB, IHC (p) and EIA.

Quick Overview for SIRT1 antibody (Internal Region) (ABIN615980)

Target: SIRT1 (Sirtuin 1 (SIRT1))

Reactivity: Human, Mouse

Host: Goat

Clonality: Polyclonal

Conjugate: This SIRT1 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Enzyme Immunoassay (EIA)

Product Details anti-SIRT1 Antibody

Binding Specificity: AA 577-590, Internal Region

Sequence: C-EKPQEVQTSR NVES

Specificity: This antibody is expected to recognize both reported isoforms (NP_036370.2 and NP_001135970.1).

Cross-Reactivity (Details): Species reactivity (expected):Mouse.
Species reactivity (tested):Human.

Purification: Ammonium Sulphate Precipitation followed by antigen Affinity Chromatography using the immunizing peptide.

Immunogen: Peptide with sequence from the internal region of the protein sequence according to NP_036370.2, NP_001135970.1. Genename: SIRT1

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Restrictions: For Research Use only

Handling

Concentration: 0.5 mg/mL

Buffer: Tris saline, pH ~7.3, 0.02 % Sodium Azide, 0.5 % BSA

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C/-20 °C

Storage Comment: Store undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.

Target Details for SIRT1

Target: SIRT1 (Sirtuin 1 (SIRT1))

Alternative Name: SIRT1

Background: The SIRT family of genes are highly conserved from prokaryotes to eukaryotes and are involved in diverse processes including transcriptional regulation, cell cycle progression, DNA damage repair and aging. Sirtuins utilize NAD+ as a substrate to catalyze deacetylation of specific acetylated protein and histones. SIRT1 has been shown to be induced by caloric restriction (CR) in several tissues and SIRT1 mediated deacetylase activity attenuates B-amyloid content in the brain during CR. SIRT1 also regulates the p53-dependent DNA damage response pathway by binding to and deacetylating p53.Synonyms: NAD-dependent deacetylase sirtuin-1, S. cerevisiae, SIR2-like protein 1, SIR2L1, SIR2alpha, SIRT-1, homolog) 1, sirtuin (silent mating type information regulation 2, sirtuin 1, sirtuin type 1, sirtuin-1

Gene ID: 23411

NCBI Accession: NP_001135970

UniProt: Q96EB6

Pathways: MAPK Signaling, Intracellular Steroid Hormone Receptor Signaling Pathway, Regulation of Intracellular Steroid Hormone Receptor Signaling, Carbohydrate Homeostasis, Positive Regulation of Endopeptidase Activity, Regulation of Carbohydrate Metabolic Process, Positive Regulation of Response to DNA Damage Stimulus, Negative Regulation of intrinsic apoptotic Signaling