SIRT1 antibody (AA 112-311)

Catalog No. ABIN3042483

Product Details anti-SIRT1 Antibody

Target: SIRT1 (Sirtuin 1 (SIRT1))

Binding Specificity: AA 112-311

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This SIRT1 antibody is un-conjugated

Application: Western Blotting (WB)

Purpose: Rabbit IgG polyclonal antibody for NAD-dependent protein deacetylase sirtuin-1(SIRT1) detection. Tested with WB in Human.

Cross-Reactivity (Details): No cross reactivity with other proteins.

Characteristics: Rabbit IgG polyclonal antibody for NAD-dependent protein deacetylase sirtuin-1(SIRT1) detection. Tested with WB in Human.
Gene Name: sirtuin 1
Protein Name: NAD-dependent protein deacetylase sirtuin-1

Purification: Immunogen affinity purified.

Immunogen: E.coli-derived human SIRT1 recombinant protein (Position: R112-K311). Human SIRT1 shares 90% amino acid (aa) sequence identity with mouse SIRT1.

Isotype: IgG

Application Details

Application Notes: WB: Concentration: 0.1-0.5 μg/mL, Tested Species: Human, The detection limit for SIRT1 is approximately 0.25 ng/lane under reducing conditions.
Notes: Tested Species: Species with positive results.
Other applications have not been tested. Optimal dilutions should be determined by end users.

Comment:

Antibody can be supported by chemiluminescence kit ABIN921124 in WB.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Concentration: 500 μg/mL

Buffer: Each vial contains 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C/-20 °C

Storage Comment: At -20°C for one year. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20 °C for a longer time. Avoid repeated freezing and thawing.

References for anti-SIRT1 antibody (ABIN3042483)

Hou, Zheng, Li, Gao, Zhang: "The protective effect of glycyrrhizic acid on renal tubular epithelial cell injury induced by high glucose." in: International journal of molecular sciences, Vol. 15, Issue 9, pp. 15026-43, (2015) (PubMed).

Target Details for SIRT1

Target: SIRT1 (Sirtuin 1 (SIRT1))

Alternative Name: SIRT1 (SIRT1 Products)

Synonyms: sirtuin 1 antibody, AA673258 antibody, SIR2L1 antibody, Sir2 antibody, Sir2a antibody, Sir2alpha antibody, sir2l1 antibody, sirtuin antibody, SIR2 antibody, sirtuin 1 antibody, sirtuin 1 L homeolog antibody, SIRT1 antibody, SRT1 antibody, Sirt1 antibody, sirt1.L antibody

Background: Sirtuin 1, also known as SIR2L1 or SIRT1, is a protein that in humans is encoded by the SIRT1 gene. It is mapped to 10q21.3. Sirtuin 1 is a member of the sirtuin family of proteins, homologs of the Sir2 gene in S. cerevisiae. Members of the sirtuin family are characterized by a sirtuin core domain and grouped into four classes. Sirtuin 1 is downregulated in cells that have high insulin resistance and inducing its expression increases insulin sensitivity, suggesting the molecule is associated with improving insulin sensitivity. Furthermore, Sirtuin 1 was shown to de-acetylate and affect the activity of both members of the PGC1-alpha/ERR-alpha complex, which are essential metabolic regulatory transcription factors.

Synonyms: 75SirT1 antibody|BA57G10.4 antibody|hSIR2 antibody|hSIRT1 antibody|HST2, S. cerevisiae, homolog of antibody|NAD dependent deacetylase SIRT1 antibody|NAD dependent deacetylase sirtuin 1 antibody|NAD dependent protein deacetylase sirtuin 1 antibody|OTTHUMP00000198111 antibody|OTTHUMP00000198112 antibody|Regulatory protein SIR2 homolog 1 antibody|SIR1_HUMAN antibody|SIR2 like 1 antibody|SIR2 like protein 1 antibody|SIR2, S.cerevisiae, homolog-like 1 antibody|SIR2-like protein 1 antibody|SIR2ALPHA antibody|SIR2alpha protein antibody|SIR2L1 antibody|SIRT 1 antibody|Sirt1 antibody|SIRT1 Sir2 like proteins (siruitins) type 1 antibody|SIRT1: sirtuin (silent mating type information regulation 2 homolog) 1 (S. cerevisiae) antibody|SirtT1 75 kDa fragment antibody|sirtuin (silent mating type information regulation 2 homolog) 1 (S. cerevisiae) antibody|Sirtuin 1 antibody|sirtuin antibody|Sirtuin type 1 antibody

Gene ID: 23411

Pathways: MAPK Signaling, Intracellular Steroid Hormone Receptor Signaling Pathway, Regulation of Intracellular Steroid Hormone Receptor Signaling, Carbohydrate Homeostasis, Positive Regulation of Endopeptidase Activity, Regulation of Carbohydrate Metabolic Process, Positive Regulation of Response to DNA Damage Stimulus, Negative Regulation of intrinsic apoptotic Signaling