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Pleckstrin Homology-Like Domain, Family A, Member 3 (PHLDA3) (C-Term) antibody

Details for Product No. ABIN615985, Supplier: Log in to see
Antigen
  • TIH1
  • Tih1
  • si:dz182n13.5
  • zgc:92333
Epitope
C-Term
11
4
2
1
1
1
Reactivity
Human, Mouse (Murine), Rat (Rattus)
27
12
11
Host
Goat
14
12
1
Clonality
Polyclonal
Conjugate
Un-conjugated
1
1
1
1
1
1
Application
Enzyme Immunoassay (EIA), Western Blotting (WB)
19
18
8
1
Supplier
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Immunogen synthetic peptide corresponding to the C Terminus of the protein sequence of Huuman PHLDA3 (NP_036528.1). Genename: PHLDA3
Sequence C-RARQSLGTGT LVS
Specificity Recognizes PHLDA3 / TIH1
Cross-Reactivity (Details) Species reactivity (expected):Mouse and Rat.
Species reactivity (tested):Human.
Purification Ammonium Sulphate Precipitation followed by antigen Affinity Chromatography using the immunizing peptide.
Alternative Name PHLDA3 (PHLDA3 Antibody Abstract)
Background P53/TP53-regulated repressor of Akt/AKT1 signaling. Represses AKT1 by preventing AKT1-binding to membrane lipids, thereby inhibiting AKT1 translocation to the cellular membrane and activation. Contributes to p53/TP53-dependent apoptosis by repressing AKT1 activity. Its directs transcription regulation by p53/TP53 may explain how p53/TP53 can negatively regulate AKT1. May acts as a tumor suppressor. The PH domain binds phosphoinositides with a broad specificity. It competes with the PH domain of AKT1 and directly interferes with AKT1 binding to phosphatidylinositol 4,5-bisphosphate (PIP2) and phosphatidylinositol 3,4,5-triphosphate (PIP3), preventing AKT1 association to membrane lipids and subsequent activation of AKT1 signaling.Synonyms: Pleckstrin homology-like domain family A member 3, TDAG51/Ipl homologue 1, TIH1
Gene ID 23612
NCBI Accession NP_036528
UniProt Q9Y5J5
Application Notes Optimal working dilution should be determined by the investigator.
Restrictions For Research Use only
Concentration 0.5 mg/mL
Buffer Tris saline, pH 7.3 containing 0.02 % Sodium Azide as preservative and 0.5 % BSA as stabilizer.
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Avoid repeated freezing and thawing.
Storage 4 °C/-20 °C
Storage Comment Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.
Supplier Images
Western Blotting (WB) image for anti-Pleckstrin Homology-Like Domain, Family A, Member 3 (PHLDA3) (C-Term) antibody (ABIN615985) HEK293 lysate (10 µg protein in RIPA buffer) overexpressing Human PHLDA3 with C-termi...
Background publications Frank, Mendelsohn, Ciccone et al.: "A novel pleckstrin homology-related gene family defined by Ipl/Tssc3, TDAG51, and Tih1: tissue-specific expression, chromosomal location, and parental imprinting." in: Mammalian genome : official journal of the International Mammalian Genome Society, Vol. 10, Issue 12, pp. 1150-9, 2000 (PubMed).