Rabbit anti-Goat IgG (Fc Region) Antibody (HRP)
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- Target See all IgG products
- IgG
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Binding Specificity
- Fc Region
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Reactivity
- Goat
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Host
- Rabbit
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Clonality
- Polyclonal
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Conjugate
- HRP
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Application
- ELISA, Immunohistochemistry (IHC), Western Blotting (WB)
- Characteristics
- Rabbit anti-goat IgG Fc is a secondary antibody to goat Fc part conjugated to HRP. Antibodies are affinity purified using solid phase goat IgG.
- Purification
- Affinity purification
- Isotype
- IgG
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- Application Notes
- 1:500-1: 5000 for immunohistochemistry (IHC),1:10 000-1:50 000 for ELISA/western blot-
- Restrictions
- For Research Use only
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- Format
- Lyophilized
- Reconstitution
- For reconstitution add 1.1 mL of sterile water. Let it stand 30 minutes at room temperature to dissolve. Prepare fresh working dilutions daily.
- Handling Advice
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Use of Sodium Azide will inhibit enzyme activity of horseradish peroxidase.
Prepare working dilution prior to use and then discard. Be sure to mix well but without foaming.
A solution with 50 % glycerol will not freeze in -20 °C. If you are using a 1:5000 dilution prior to diluting with glycerol, then you would need to use a 1:2500 dilution after adding glycerol. - Storage
- 4 °C
- Storage Comment
- Store lyophilized material at 2-8°C.For long time storage after reconstitution, dilute the antibody solution with glycerol to a final concentration of 50% glycerol and store as liquid at -20°C, to prevent loss of enzymatic activity. For example, if you have reconstituted 1 mg of antibody in 1.1 ml of sterile water add 1.1 ml of glycerol. Such solution will not freeze in -20°C. If you are using a 1:5000 dilution prior to diluting with glycerol, then you would need to use a 1:2500 dilution after adding glycerol. Prepare working dilution prior to use and then discard. Be sure to mix well but without foaming.
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- Target
- IgG
- Abstract
- IgG Products
- Target Type
- Antibody
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