Rabbit anti-Goat IgG (Fc Region) Antibody (HRP)

Catalog No. ABIN618944

Product Details

Target: IgG

Binding Specificity: Fc Region

Reactivity: Goat

Host: Rabbit

Clonality: Polyclonal

Conjugate: HRP

Application: ELISA, Immunohistochemistry (IHC), Western Blotting (WB)

Characteristics: Rabbit anti-goat IgG Fc is a secondary antibody to goat Fc part conjugated to HRP. Antibodies are affinity purified using solid phase goat IgG.

Purification: Affinity purification

Isotype: IgG

Application Details

Application Notes: 1:500-1: 5000 for immunohistochemistry (IHC),1:10 000-1:50 000 for ELISA/western blot-

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: For reconstitution add 1.1 mL of sterile water. Let it stand 30 minutes at room temperature to dissolve. Prepare fresh working dilutions daily.

Handling Advice: Use of Sodium Azide will inhibit enzyme activity of horseradish peroxidase.
Prepare working dilution prior to use and then discard. Be sure to mix well but without foaming.
A solution with 50 % glycerol will not freeze in -20 °C. If you are using a 1:5000 dilution prior to diluting with glycerol, then you would need to use a 1:2500 dilution after adding glycerol.

Storage: 4 °C

Storage Comment: Store lyophilized material at 2-8°C.For long time storage after reconstitution, dilute the antibody solution with glycerol to a final concentration of 50% glycerol and store as liquid at -20°C, to prevent loss of enzymatic activity. For example, if you have reconstituted 1 mg of antibody in 1.1 ml of sterile water add 1.1 ml of glycerol. Such solution will not freeze in -20°C. If you are using a 1:5000 dilution prior to diluting with glycerol, then you would need to use a 1:2500 dilution after adding glycerol. Prepare working dilution prior to use and then discard. Be sure to mix well but without foaming.

Target Details

Target: IgG

Abstract: IgG Products

Target Type: Antibody