Caspase 3, Apoptosis-Related Cysteine Peptidase (CASP3) (AA 149-179), (Asp175) antibody

Details for Product No. ABIN651176
Request Want additional data for this product?

The Independent Validation Initiative strives to provide you with high quality data. Find out more

Antigen
Synonyms
CC3, CG14902, Cas3, Casp 3, Casp3, Caspase-3, DECAY, Decay, Dmel\\CG14902, Drosophila executioner caspase related to Apopain/Yama, caspase 3, caspase-3, CG7788, DRICE, Dmel\\CG7788, DrICE, DrIce, Dric ... show more
CC3, CG14902, Cas3, Casp 3, Casp3, Caspase-3, DECAY, Decay, Dmel\\CG14902, Drosophila executioner caspase related to Apopain/Yama, caspase 3, caspase-3, CG7788, DRICE, Dmel\\CG7788, DrICE, DrIce, Drice, ICE, crice, drICE, drIce, drice, ice, A830040C14Rik, AC-3, Apopain, CPP32, Lice, Yama, mldy, CPP32B, SCA-1, xcpp32 show less
Epitope
AA 149-179, Asp175
(28), (22), (20), (15), (14), (12), (8), (8), (8), (8), (7), (7), (7), (6), (6), (6), (6), (6), (5), (5), (4), (3), (3), (3), (3), (2), (2), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Reactivity
Human
(308), (118), (94), (19), (18), (18), (6), (5), (4), (4), (4), (3), (2), (2), (2), (1)
Host
Rabbit
(275), (60), (7), (2), (2)
Clonality (Clone)
Polyclonal ()
Conjugate
Un-conjugated
(10), (8), (6), (4), (4), (4), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Application
Western Blotting (WB), Immunohistochemistry (IHC), Flow Cytometry (FACS)
(263), (118), (99), (91), (54), (31), (26), (19), (19), (14), (10), (4), (2), (2), (2)
Pubmed 1 reference available
Quantity 400 µL
Options
Shipping to United States (Change)
Availability Will be delivered in 2 to 3 Business Days
Request Want additional data for this product?

The Independent Validation Initiative strives to provide you with high quality data. Find out more

Catalog No. ABIN651176
291.50 $
Plus shipping costs $45.00

Order hotline:

  • +1 404 474 4654
  • +1 888 205 9894 (TF)
Immunogen This CASP3(Asp175) antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 149-179 AA from human CASP3(Asp175).
Clone RB25834
Isotype Ig
Specificity This CASP3(Asp175) antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between amino acid 155-185 from the Asp175 region of human CASP3.
Purification This antibody is purified through a protein A column, followed by peptide affinity purification.
Alternative Name CASP3
Background This gene encodes a protein which is a member of the cysteine-aspartic acid protease (caspase) family. Sequential activation of caspases plays a central role in the execution-phase of cell apoptosis. Caspases exist as inactive proenzymes which undergo proteolytic processing at conserved aspartic residues to produce two subunits, large and small, that dimerize to form the active enzyme. This protein cleaves and activates caspases 6, 7 and 9, and the protein itself is processed by caspases 8, 9 and 10. It is the predominant caspase involved in the cleavage of amyloid-beta 4A precursor protein, which is associated with neuronal death in Alzheimer's disease. Alternative splicing of this gene results in two transcript variants that encode the same protein.
Synonyms: Caspase-3 subunit p12, Caspase-3 subunit p17, SREBP cleavage activity 1, SCA-1, Protein Yama, Cysteine protease CPP32, CPP-32, Apopain, Caspase-3, CASP-3,CPP32, CASP3
Molecular Weight 31608 DA
Gene ID 836
NCBI Accession NP_004337
Research Area Apoptosis/Necrosis, Signaling, Phospho-specific antibodies, Protein Modifications, Metabolism, Cell Structure
Application Notes WB = 1:1000, IHC = 1:50-100, FACS = 1:10-50
Restrictions For Research Use only
Format Liquid
Concentration 0.33 mg/mL
Buffer PBS with 0.09 % (W/V) sodium azide
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage 4 °C/-20 °C
Storage Comment Maintain refrigerated at 2-8 °C for up to 6 months. For long term storage store at -20 °C in small aliquots to prevent freeze-thaw cycles.
Expiry Date 6 months
Supplier Images
anti-Caspase 3, Apoptosis-Related Cysteine Peptidase (CASP3) (AA 149-179), (Asp175) antibody Western blot analysis of CASP3 (Asp175) Antibody (ABIN651176) in CEM cell line lysates (35 µg/lane). CASP3 (arrow) was detected using the purified polyclonal antibody.
anti-Caspase 3, Apoptosis-Related Cysteine Peptidase (CASP3) (AA 149-179), (Asp175) antibody (2) CASP3(Asp175) Antibody (ABIN651176) IHC analysis in formalin fixed and paraffin embedded human brain tissue followed by peroxidase conjugation of the secondary antibody and DAB staining
anti-Caspase 3, Apoptosis-Related Cysteine Peptidase (CASP3) (AA 149-179), (Asp175) antibody (3) Western blot analysis of CASP3 (arrow) using rabbit polyclonal CASP3 Antibody (ABIN651176). 293 cell lysates (2 µg/lane) either nontransfected (Lane 1) or transiently transfected (Lane 2) with the CASP3 gene.
Background publications Mei, Yong, Liu et al.: "tRNA binds to cytochrome c and inhibits caspase activation." in: Molecular cell, Vol. 37, Issue 5, pp. 668-78, 2010 (PubMed).

Validation Images
Did you look for something else?
back to top