Endonuclease VIII-Like 1 (NEIL1) (Center) antibody

Antigen: Endonuclease VIII-Like 1 (NEIL1)

Synonyms: FPG1, NEI1, hFPG1, FLJ22402

Binding Site: Center

Clonality: Polyclonal

Host: Rabbit

Reactivity: Human

Application: Western Blotting (WB), ELISA

Certificates: ISO 9001:2008

Catalog no.: ABIN653649

Additional Information

Alternative name: NEIL1

Gene ID: 79661

UniProt: Q96FI4

Immunogen: This NEIL1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 137~167 amino acids from the Central region of human NEIL1.

Isotype: Ig

Description: Other names: FPG1, Nei homolog 1, NEH1, Nei-like protein 1, DNA-(apurinic or apyrimidinic site) lyase Neil1, DNA glycosylase/AP lyase Neil1, Endonuclease VIII-like 1, NEIL1

Characteristics: Purified Rabbit Polyclonal Antibody (Pab)

Specificity: This NEIL1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 137~167 amino acids from the Central region of human NEIL1.

Molecular Weight: 43684 DA

Comments:

Background: NEIL1 belongs to a class of DNA glycosylases homologous to the bacterial Fpg/Nei family. These glycosylases initiate the first step in base excision repair by cleaving bases damaged by reactive oxygen species and introducing a DNA strand break via the associated lyase reaction (Bandaru et al., 2002 [PubMed 12509226]).

Application Details

Application Notes: The suggested dilution is: ELISA ~~ 1:1,000 Western blotting~~ 1:50~100

Concentration: 0.25 mg/ml

Purification: Purified

Buffer: Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.

Storage: Maintain refrigerated at 2-8 deg C for up to 6 months. For long term storage store at -20 deg C in small aliquots to prevent freeze-thaw cycles

Research Area: Cancer, Cell Structure

Restrictions: For Research Use only

Images

Western blot analysis of NEIL1 Antibody (Center) (ABIN653649) in A2058 cell line lysates (35ug/lane). NEIL1 (arrow) was detected using the purified Pab.

Publications

Product: Horikawa, Wood, Yang et al.: "Single nucleotide polymorphisms of microRNA machinery genes modify the risk of renal cell carcinoma." in: Clinical cancer research : an official journal of the American Association for Cancer Research, Vol. 14, Issue 23, pp. 7956-62, 2008 (PubMed).

Muftuoglu, de Souza-Pinto, Dogan et al.: "Cockayne syndrome group B protein stimulates repair of formamidopyrimidines by NEIL1 DNA glycosylase." in: The Journal of biological chemistry, Vol. 284, Issue 14, pp. 9270-9, 2009 (PubMed).

Zhao, Krishnamurthy, Burrows et al.: "Mutation versus repair: NEIL1 removal of hydantoin lesions in single-stranded, bulge, bubble, and duplex DNA contexts." in: Biochemistry, Vol. 49, Issue 8, pp. 1658-66, 2010 (PubMed).