KDM3B Lysine (K)-Specific Demethylase 3B (KDM3B) (C-Term), (AA 1328-1358) antibody

Details for Product No. ABIN655393
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Antigen
Epitope
C-Term, AA 1328-1358
(2), (1), (1), (1)
Reactivity
Mouse (Murine)
(2), (1)
Host
Rabbit
(3)
Clonality (Clone)
Polyclonal ()
Application
Western Blotting (WB), Flow Cytometry (FACS)
(3), (3)
Pubmed 1 reference available
Quantity 400 μL
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Catalog No. ABIN655393
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Immunogen This JHDM2b antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 1328-1358 AA from the C-terminal region of human JHDM2b.
Clone RB14629
Isotype Ig
Specificity This JHDM2b antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 1335-1358 amino acids from the C-terminal region of human JHDM2b.
Purification This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.
Alternative Name JHDM2b
Background Histone demethylase that specifically demethylates 'Lys-9' of histone H3, thereby playing a central role in histone code. Demethylation of Lys residue generates formaldehyde and succinate. May have tumor suppressor activity.
Synonyms: C5orf7, JHDM2B, JMJD1B, KIAA1082
Molecular Weight 191,581 DA
Gene ID 51780
NCBI Accession NP_057688
UniProt Q7LBC6
Research Area Cell Structure
Application Notes WB = 1:1000, FACS = 1:10-50
Restrictions For Research Use only
Format Liquid
Concentration 2 mg/mL
Buffer PBS with 0.09 % (W/V) sodium azide
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage 4 °C/-20 °C
Storage Comment Maintain refrigerated at 2-8 °C for up to 6 months. For long term storage store at -20 °C in small aliquots to prevent freeze-thaw cycles.
Expiry Date 6 months
Supplier Images
anti-KDM3B Lysine (K)-Specific Demethylase 3B (KDM3B) (C-Term), (AA 1328-1358) antibody JHDM2b Antibody (C-term) western blot analysis in mouse heart tissue lysates (35ug/lane).This demonstrates the JHDM2b antibody detected the JHDM2b protein (arrow).
anti-KDM3B Lysine (K)-Specific Demethylase 3B (KDM3B) (C-Term), (AA 1328-1358) antibody (2) JHDM2b Antibody (C-term) flow cytometric analysis of Hela cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
Background publications Guo, Huss, Tong et al.: "Resolution of cell fate decisions revealed by single-cell gene expression analysis from zygote to blastocyst." in: Developmental cell, Vol. 18, Issue 4, pp. 675-85, 2010 (PubMed).

Validation Images
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