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Lin-28 Homolog A (C. Elegans) (LIN28A) antibody

Details for Product No. ABIN655496, Supplier: Log in to see
Antigen
  • Lin28
  • csdd1
  • lin-28
  • lin28
  • zcchc1
  • LIN28
  • CSDD1
  • LIN-28
  • ZCCHC1
  • lin-28A
  • AL024421
  • ENSMUSG00000070700
  • Gm10299
  • Lin-28
  • Tex17
  • RGD1566408
  • sb:cb650
  • zgc:55584
Reactivity
Human, Mouse (Murine)
135
43
28
1
1
1
Host
Rabbit
106
38
Clonality (Clone)
Polyclonal ()
Conjugate
Un-conjugated
5
5
5
4
3
3
1
1
1
1
1
1
1
1
1
Application
Flow Cytometry (FACS), Western Blotting (WB)
99
59
50
35
20
18
17
15
11
11
7
1
1
1
Supplier
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Immunogen This LIN28A antibody is generated from rabbits immunized with a His fusion protein from human LIN28A.
Clone RB14740
Isotype Ig
Specificity This LIN28A antibody is generated from rabbits immunized with a His fusion protein from human LIN28A.
Purification This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.
Alternative Name LIN28 (LIN28A Antibody Abstract)
Background Acts as a 'translational enhancer', driving specific mRNAs to polysomes and thus increasing the efficiency of protein synthesis. Its association with the translational machinery and target mRNAs results in an increased number of initiation events per molecule of mRNA and, indirectly, in stabilizing the mRNAs. Binds IGF2 mRNA, MYOD1 mRNA, ARBP/36B4 ribosomal protein mRNA and its own mRNA. Essential for skeletal muscle differentiation program through the translational up-regulation of IGF2 expression (By similarity). Acts as a suppressor of microRNA (miRNA) biogenesis by specifically binding the precursor let-7 (pre-let-7), a miRNA precursor. Acts by binding pre-let-7 and recruiting ZCCHC11/TUT4 uridylyltransferase, leading to the terminal uridylation of pre-let-7. Uridylated pre-let-7 miRNAs fail to be processed by Dicer and undergo degradation. Degradation of pre-let-7 in embryonic stem (ES) cells contributes to the maintenance of ES cells. In contrast, LIN28A down-regulation in neural stem cells by miR-125, allows the processing of pre-let-7. Specifically recognizes the 5'-GGAG-3' motif in the terminal loop of pre-let-7. Also recognizes and binds non pre-let-7 pre-miRNAs that contain the 5'-GGAG-3' motif in the terminal loop, leading to their terminal uridylation and subsequent degradation.
Synonyms: Protein lin-28 homolog A,LIN28A,CSDD1, LIN28, ZCCHC1
Molecular Weight 22743 DA
Gene ID 79727
NCBI Accession NP_078950
UniProt Q9H9Z2
Research Area Cell Structure, Signaling, Chromatin
Application Notes WB = 1:1000, FACS = 1:10-50
Restrictions For Research Use only
Format Liquid
Concentration 2 mg/mL
Buffer PBS with 0.09 % (W/V) sodium azide
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage 4 °C/-20 °C
Storage Comment Maintain refrigerated at 2-8 °C for up to 6 months. For long term storage store at -20 °C in small aliquots to prevent freeze-thaw cycles.
Expiry Date 6 months
Supplier Images
Western Blotting (WB) image for anti-Lin-28 Homolog A (C. Elegans) (LIN28A) antibody (ABIN655496) LIN28 Antibody western blot analysis in MDA-MB435 cell line lysates (35ug/lane).This...
Western Blotting (WB) image for anti-Lin-28 Homolog A (C. Elegans) (LIN28A) antibody (ABIN655496) LIN28 Antibody western blot analysis in mouse Neuro-2a cell line lysates (35ug/lane)...
Flow Cytometry (FACS) image for anti-Lin-28 Homolog A (C. Elegans) (LIN28A) antibody (ABIN655496) LIN28 Antibody flow cytometric analysis of MDA-MB435 cells (right histogram) compare...
Background publications Peng, Maihle, Huang: "Pluripotency factors Lin28 and Oct4 identify a sub-population of stem cell-like cells in ovarian cancer." in: Oncogene, Vol. 29, Issue 14, pp. 2153-9, 2010 (PubMed).

Qiu, Ma, Wang et al.: "Lin28-mediated post-transcriptional regulation of Oct4 expression in human embryonic stem cells." in: Nucleic acids research, Vol. 38, Issue 4, pp. 1240-8, 2010 (PubMed).

Iliopoulos, Hirsch, Struhl: "An epigenetic switch involving NF-kappaB, Lin28, Let-7 MicroRNA, and IL6 links inflammation to cell transformation." in: Cell, Vol. 139, Issue 4, pp. 693-706, 2009 (PubMed).

Heo, Joo, Kim et al.: "TUT4 in concert with Lin28 suppresses microRNA biogenesis through pre-microRNA uridylation." in: Cell, Vol. 138, Issue 4, pp. 696-708, 2009 (PubMed).

West, Viswanathan, Yabuuchi et al.: "A role for Lin28 in primordial germ-cell development and germ-cell malignancy." in: Nature, Vol. 460, Issue 7257, pp. 909-13, 2009 (PubMed).