MTOR antibody (Mechanistic Target of Rapamycin (serine/threonine Kinase)) (AA 2436-2492)

Details for Product anti-FRAP1 Antibody No. ABIN676403, Supplier: Log in to see
Antigen
  • FRAP1
  • FRAP
  • FRAP2
  • RAFT1
  • RAPT1
  • 2610315D21Rik
  • AI327068
  • Frap1
  • flat
  • frap1
  • tor
  • wu:fc22h08
  • mechanistic target of rapamycin (serine/threonine kinase)
  • MTOR
  • Mtor
  • mtor
Alternatives
anti-Human MTOR antibody for Immunofluorescence (fixed cells)
Epitope
AA 2436-2492
64
28
26
22
21
20
15
14
12
10
9
5
4
4
4
3
3
3
2
2
2
2
2
2
2
2
2
2
1
1
1
1
1
1
1
1
1
1
1
1
1
1
Reactivity
Human, Mouse (Murine), Rat (Rattus)
367
199
197
18
9
8
7
7
7
7
4
2
2
2
1
1
Host
Rabbit
315
48
3
1
Clonality
Polyclonal
Conjugate
This MTOR antibody is un-conjugated
8
8
7
5
4
4
4
4
4
4
4
4
4
4
4
4
3
3
Application
Immunofluorescence (Paraffin-embedded Sections) (IF (p)), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))
246
110
94
71
54
51
37
19
7
7
7
6
3
2
2
2
1
1
1
Options
Supplier
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Available images

'Independent Validation' Badge
Lot Number 131209
Method validated Western Blotting
Positive Control HeLa cells
Negative Control c6/36 mosquito cells (non-reactive species)
Notes A strong band was observed in the positive control sample at the correct molecular weight. Multiple faint bands were also observed in the positive sample, but these bands did not represent a significant background signal. A band at the correct molecular weight was also observed in the negative control; however, it may be impossible to find a true negative control for this antigen.
Primary Antibody
  • Antigen: Mechanistic Target of Rapamycin (serine/threonine Kinase) (FRAP1) (1:200 dilution)
  • Catalog number: ABIN676403
  • Supplier: Log in to see
  • Supplier catalog number: Log in to see
  • Lot number: 131209
Secondary Antibody
Controls
  • Positive control: HeLa cell extract
  • Negative control: c6/36 Mosquito cell extract
Protocol
  • Total protein extracts were boiled in 1X SDS Sample Buffer containing 1% SDS and 1.25% β-mercaptoethanol at 95°C for 5 min prior to loading.
  • 20 μg of boiled extracts were loaded and resolved on 8-16% SDS-polyacrylamide gel.
  • The Thermo Scientific - Spectra Multicolor Broad Range (Cat # 26634) were used as molecular mass markers.
  • Proteins were then transferred onto PVDF membrane by wet transfer and protein transfer was confirmed with Ponceau-S staining.
  • The PVDF membrane was incubated with 25 mL of blocking buffer [Tris Buffered Saline, pH 7.4 plus 0.1% TW20 (TBST)] containing 5% (W/V) BSA at room temperature for 1 h.
  • The membrane was rinsed with TBST once.
  • The membrane was immersed with the protein side up in the primary antibody solution (FRAP1; 1:200) in TBST containing 5% (W/V) BSA and incubated for 16 h at 4°C.
  • The membrane was rinsed in TBST thrice for 5 min each.
  • The membrane was incubated in the HRP-conjugated secondary antibody solution (Goat anti-rabbit IgG-HRP; 1:20,000) in TBST containing 5% (W/V) BSA and incubated for 1 h at room temperature (~26°C) with gentle agitation.
  • The membrane was rinsed thrice TBST thrice for 5 min each.
  • The membrane was rinsed in TBS twice for 30 s each.
  • Signals were detected with ECL-2 Substrate. The blot was scanned for 300 s.
  • The membrane was rinsed three times TBST.
  • Incubated in Acidic Glycine Stripping Buffer at room temperature with gentle agitation for 3 times, 10 min each.
  • The membrane was washed in TBST 2 times for 10 min each.
  • Repeated Steps 5-12 with the loading control antibody (anti-Actin; 1:6,000) and its matching secondary antibody (Goat anti-rabbit IgG-HRP; 1:20,000).
Experimental Notes - No experimental challenges noted.
Validation Images
Western Blotting validation image for anti-Mechanistic Target of Rapamycin (serine/threonine Kinase) (FRAP1) (AA 2436-2492) antibody (ABIN676403) Figure 1. Western blot of lysates from HeLa cells (Lane 1) and c6/36 cells (Lane 2) p...
Immunogen KLH conjugated synthetic peptide derived from human mTOR
Isotype IgG
Purification Purified by Protein A.
Alternative Name Mtor/Frap (FRAP1 Antibody Abstract)
Background

The protein encoded by this gene belongs to a family of phosphatidylinositol kinase-related kinases. These kinases mediate cellular responses to stresses such as DNA damage and nutrient deprivation. This protein acts as the target for the cell-cycle arrest and immunosuppressive effects of the FKBP12-rapamycin complex. The ANGPTL7 gene is located in an intron of this gene. [provided by RefSeq, Sep 2008]

Synonyms: Serine/threonine-protein kinase mTOR, FK506-binding protein 12-rapamycin complex-associated protein 1, FKBP12-rapamycin complex-associated protein, Mammalian target of rapamycin, mTOR, Mechanistic target of rapamycin, Rapamycin and FKBP12 target 1, Rapamycin target protein 1, MTOR, FRAP, FRAP1, FRAP2, RAFT1, RAPT1

Gene ID 2475
UniProt P42345
Pathways PI3K-Akt Signaling, RTK Signaling, AMPK Signaling, Interferon-gamma Pathway, Fc-epsilon Receptor Signaling Pathway, EGFR Signaling Pathway, Neurotrophin Signaling Pathway, Regulation of Actin Filament Polymerization, Regulation of Muscle Cell Differentiation, Regulation of Cell Size, Skeletal Muscle Fiber Development, Regulation of Carbohydrate Metabolic Process, Autophagy, CXCR4-mediated Signaling Events
Application Notes IHC-P 1:100-500
IF(IHC-P) 1:50-200
Restrictions For Research Use only
Format Liquid
Concentration 1 μg/μL
Buffer Aqueous buffered solution containing 1 % BSA, 50 % glycerol and 0.09 % sodium azide.
Preservative Sodium azide
Precaution of Use This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.
Storage -20 °C
Storage Comment Store at -20°C
Expiry Date 12 months
Supplier Images
Immunofluorescence (IF) image for anti-MTOR antibody (Mechanistic Target of Rapamycin (serine/threonine Kinase)) (AA 2436-2492) (ABIN676403) Formalin-fixed and paraffin embedded mouse melanoma tissue labeled with Anti-mTOR Pol...
Immunohistochemistry (IHC) image for anti-MTOR antibody (Mechanistic Target of Rapamycin (serine/threonine Kinase)) (AA 2436-2492) (ABIN676403) Formalin-fixed and paraffin embedded rat testis tissue labeled with Anti-mTOR Polyclo...
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) image for anti-MTOR antibody (Mechanistic Target of Rapamycin (serine/threonine Kinase)) (AA 2436-2492) (ABIN676403) Formalin-fixed and paraffin embedded mouse melanoma tissue labeled with Anti-mTOR Pol...
Product cited in: Zou, Geng, Chen, Ren, Liu, Wan, Guo, Wang: "Ranolazine improves oxidative stress and mitochondrial function in the atrium of acetylcholine-CaCl2 induced atrial fibrillation rats." in: Life sciences, Vol. 156, pp. 7-14, 2016 (PubMed).

Madka, Mohammed, Li, Zhang, Biddick, Patlolla, Lightfoot, Towner, Wu, Steele, Kopelovich, Rao: "Targeting mTOR and p53 Signaling Inhibits Muscle Invasive Bladder Cancer In Vivo." in: Cancer prevention research (Philadelphia, Pa.), Vol. 9, Issue 1, pp. 53-62, 2016 (PubMed).

Yang, Wang, Wang, Zhang, Zhang, Lu, Wang: "mTOR is involved in 17?-estradiol-induced, cultured immature boar Sertoli cell proliferation via regulating the expression of SKP2, CCND1, and CCNE1." in: Molecular reproduction and development, Vol. 82, Issue 4, pp. 305-14, 2015 (PubMed).

Li, Liu, Wang, Sun, Ding, Sun, Han, Wang: "Follistatin could promote the proliferation of duck primary myoblasts by activating PI3K/Akt/mTOR signalling." in: Bioscience reports, Vol. 34, Issue 5, 2014 (PubMed).