ASK1 antibody (pSer966) (Cy5)

Catalog No. ABIN682802

Product Details anti-ASK1 Antibody (Cy5)

Target: ASK1 (MAP3K5) (Mitogen-Activated Protein Kinase Kinase Kinase 5 (MAP3K5))

Binding Specificity: pSer966

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This ASK1 antibody is conjugated to Cy5

Application: Western Blotting (WB)

Cross-Reactivity: Human

Predicted Reactivity: Mouse,Rat,Dog,Horse,Chicken

Purification: Purified by Protein A.

Immunogen: KLH conjugated synthetic phosphopeptide derived from human ASK1 around the phosphorylation site of Ser966

Isotype: IgG

Application Details

Application Notes: IF(IHC-P) 1:50-200

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 μg/μL

Buffer: Aqueous buffered solution containing 0.01M TBS ( pH 7.4) with 1 % BSA, 0.03 % Proclin300 and 50 % Glycerol.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Store at -20°C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles.

Expiry Date: 12 months

Target Details for ASK1

Target: ASK1 (MAP3K5) (Mitogen-Activated Protein Kinase Kinase Kinase 5 (MAP3K5))

Alternative Name: Ask1 (MAP3K5 Products)

Synonyms: ASK1 antibody, MAPKKK5 antibody, MEKK5 antibody, mitogen-activated protein kinase kinase kinase 5 antibody, MUD21.11 antibody, MUD21_11 antibody, 7420452D20Rik antibody, ASK antibody, Mekk5 antibody, Ask1 antibody, RGD1306565 antibody, mitogen-activated protein kinase kinase kinase 5 antibody, MAP3K5 antibody, MAPKKK5 antibody, Map3k5 antibody, map3k5 antibody

Background:

Synonyms: ASK1 phospho S966, p-ASK1 phospho S966, ASK1Ser966, Mitogen-activated protein kinase kinase kinase 5, MAPK/ERK kinase kinase 5, MEK kinase 5, MEKK 5, Apoptosis signal-regulating kinase 1, ASK-1, MAP3K5, ASK1, MAPKKK5, MEKK5, M3K5_HUMAN.

Background: Mitogen-activated protein kinase (MAPK) signaling cascades include MAPK or extracellular signal-regulated kinase (ERK), MAPK kinase (MKK or MEK), and MAPK kinase kinase (MAPKKK or MEKK). MAPKK kinase/MEKK phosphorylates and activates its downstream protein kinase, MAPK kinase/MEK, which in turn activates MAPK. The kinases of these signaling cascades are highly conserved, and homologs exist in yeast, Drosophila, and mammalian cells. MAPKKK5 contains 1,374 amino acids with all 11 kinase subdomains. Northern blot analysis shows that MAPKKK5 transcript is abundantly expressed in human heart and pancreas. The MAPKKK5 protein phosphorylates and activates MKK4 (aliases SERK1, MAPKK4) in vitro, and activates c-Jun N-terminal kinase (JNK)/stress-activated protein kinase (SAPK) during transient expression in COS and 293 cells, MAPKKK5 does not activate MAPK/ERK.

Gene ID: 4217

Pathways: MAPK Signaling, Positive Regulation of Endopeptidase Activity, Unfolded Protein Response