Osteoactivin (AA 85-135) antibody

Details for Product No. ABIN749093, Supplier: Log in to see
Antigen
  • HGFIN
  • NMB
  • DC-HIL
  • Dchil
  • ipd
  • glycoprotein (transmembrane) nmb
  • Gpnmb
  • GPNMB
  • gpnmb
Epitope
AA 85-135
19
18
14
11
9
8
8
7
5
4
4
3
2
2
2
1
1
1
1
1
1
Reactivity
Human, Mouse (Murine), Rat (Rattus)
121
28
23
5
5
5
4
4
2
2
2
1
1
Host
Rabbit
68
58
1
Clonality
Polyclonal
Conjugate
Un-conjugated
5
5
4
4
4
3
3
3
3
3
3
3
2
1
1
1
1
1
1
1
1
1
1
1
1
1
1
Application
Immunofluorescence (Paraffin-embedded Sections) (IF (p)), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)
77
59
37
21
15
12
4
3
3
1
Options
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Available images

'Independent Validation' Badge
Antigen Osteoactivin
Method validated Immunohistochemistry (Formalin-fixed Paraffin-embedded Sections)
Positive Control Tumor cell transplanted mouse brain was dissected
Notes The staining pattern was as expected for Osteoactivin as membrane protein.
Primary Antibody ABIN749093
Isotype Control Antibody rabbit IgG
Secondary Antibody Donkey-anti-Rabbit-Cy3
Protocol
  • Tumor cell transplanted mouse brain was dissected, fixed in 4% PFA for overnight, saturated in 30% sucrose, mounted in Cryo, and sectioned into 12µm sections.
  • Immunostaining for Cryo Sections:
    • Dry slides at 50-55°C for 15-30 min (or RT for 30min)
    • Wash three times with PBS 5min (shake gently)
    • Block with 10% Normal Donkey Serum (NDS) at RM for 1hour
    • Incubate with primary antibody at 4°C overnight (Prepared in 2% NDS)
    • Rabbit-anti-Osteoactivin antibody (ABIN749093) diluted 1:50-1:400
    • Wash three times with PBS 5min (shake gently)
    • Incubate with secondary antibody together with DAPI at RT for 1h (Prepared in 2% NDS), cover the chamber with foil
    • Donkey-anti-Rabbit-Cy3
    • DAPI 1:100
    • Wash three times with PBS 5min (shake gently)
    • Add three drops of water-based Immu-mount
    • Cover with 24x60 cover glass
  • Imaging
    • Images were taken using a Zeiss Axio2-Imaging system, 20X-0.5NA-Dry Dye-1004-072 objective, DAPI and Cy3 channel.
    Experimental Notes ABIN749093 worked within the diution range 1:50 - 1:200; the antibody did not work well at a 1:400 dilution.
    Validation Images
    Immunohistochemistry (Formalin-fixed Paraffin-embedded Sections) validation image for anti-Osteoactivin (AA 85-135) antibody (ABIN749093) IF validation of osteoactivin antibody ABIN749093 on mouse brain tumor sections. A. A...
    Immunogen KLH conjugated synthetic peptide derived from human GPNMB
    Isotype IgG
    Purification Purified by Protein A.
    Alternative Name Gpnmb
    Background

    The protein encoded by this gene is a type I transmembrane glycoprotein which shows homology to the pMEL17 precursor, a melanocyte-specific protein. GPNMB shows expression in the lowly metastatic human melanoma cell lines and xenografts but does not show expression in the highly metastatic cell lines. GPNMB may be involved in growth delay and reduction of metastatic potential. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq].

    Subcellular location: Extracellular

    Synonyms: NMB, HGFIN, Transmembrane glycoprotein NMB, Transmembrane glycoprotein HGFIN, GPNMB, UNQ1725/PRO9925

    Gene ID 10457
    UniProt Q14956
    Research Area Cancer, Innate Immunity
    Application Notes WB 1:100-1000
    IHC-P 1:100-500
    IF(IHC-P) 1:50-200
    Restrictions For Research Use only
    Format Liquid
    Concentration 1 μg/μL
    Buffer Aqueous buffered solution containing 1 % BSA, 50 % glycerol and 0.09 % sodium azide.
    Preservative Sodium azide
    Precaution of Use This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.
    Storage -20 °C
    Storage Comment Store at -20°C
    Expiry Date 12 months
    Supplier Images
    SDS-PAGE (SDS) image for anti-Osteoactivin (AA 85-135) antibody (ABIN749093) Formalin-fixed and paraffin embedded rat lung labeled with Anti-GPNMB/Osteoactivin Po...
    Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) image for anti-Osteoactivin (AA 85-135) antibody (ABIN749093) Formalin-fixed and paraffin embedded rat lung labeled with Anti-GPNMB/Osteoactivin Po...
    Product cited in: Arosarena, Dela Cadena, Denny, Bryant, Barr, Thorpe, Safadi: "Osteoactivin Promotes Migration of Oral Squamous Cell Carcinomas." in: Journal of cellular physiology, Vol. 231, Issue 8, pp. 1761-70, 2016 (PubMed).

    Frara, Abdelmagid, Tytell, Amin, Popoff, Safadi, Barbe: "Growth and repair factors, osteoactivin, matrix metalloproteinase and heat shock protein 72, increase with resolution of inflammation in musculotendinous tissues in a rat model of repetitive grasping." in: BMC musculoskeletal disorders, Vol. 17, pp. 34, 2016 (PubMed). Method employed by authors: Western Blotting (WB) (Sample species: Rat (Rattus)).

    Ruan, Li, Nie, Zhou, Zou: "Differential expression of glycoprotein non-metastatic melanoma protein B (GPNMB) involved in trichostatin A-induced apoptosis in gastric cancer." in: International journal of clinical and experimental medicine, Vol. 7, Issue 12, pp. 4857-66, 2015 (PubMed). Method employed by authors: Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) (Sample species: Human).

    Abdelmagid, Sondag, Moussa, Belcher, Yu, Stinnett, Novak, Mbimba, Khol, Hankenson, Malcuit, Safadi: "Mutation in Osteoactivin Promotes Receptor Activator of NF?B Ligand (RANKL)-mediated Osteoclast Differentiation and Survival but Inhibits Osteoclast Function." in: The Journal of biological chemistry, Vol. 290, Issue 33, pp. 20128-46, 2015 (PubMed).