Osteoactivin antibody (N-Term)

Catalog No. ABIN2781893

The Rabbit Polyclonal anti-Osteoactivin antibody (ABIN2781893) specifically detects Osteoactivin in WB and IHC. The antibody is reactive with Human, Mouse, Rat, Horse, Cow, Dog, Guinea Pig and Rabbit samples.

Quick Overview for Osteoactivin antibody (N-Term) (ABIN2781893)

Target: Osteoactivin (GPNMB)

Reactivity: Human, Mouse, Rat, Horse, Cow, Dog, Guinea Pig, Rabbit

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Osteoactivin antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC)

Product Details anti-Osteoactivin Antibody

Binding Specificity: N-Term

Purpose: GPNMB Antibody - N-terminal region

Sequence: DENDWNEKLY PVWKRGDMRW KNSWKGGRVQ AVLTSDSPAL VGSNITFAVN

Specificity: 100 % homologous to both isoforms of hGPNMB

Predicted Reactivity: Cow: 100%, Dog: 79%, Guinea Pig: 93%, Horse: 100%, Human: 100%, Mouse: 100%, Rabbit: 100%, Rat: 93%

Characteristics: This is a rabbit polyclonal antibody against GPNMB. It was validated on Western Blot and immunohistochemistry.

Purification: Affinity Purified

Immunogen: The immunogen is a synthetic peptide directed towards the N terminal region of human GPNMB

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Comment:

We also sell a specific blocking peptide that can be used in combination with this antibody. You can find the blocking peptide under ABIN8107419

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.5 mg/mL

Buffer: Purified antibody supplied in 1x PBS buffer with 0.09 % (w/v) sodium azide and 2 % sucrose.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: prevent freeze-thaw cycles

Storage: 4 °C,-20 °C

Storage Comment: For short term use, store at 2-8C up to 1 week. For long term storage, store at -20 °C in small aliquots to prevent freeze-thaw cycles.

Target Details for Osteoactivin

Target: Osteoactivin (GPNMB)

Alternative Name: GPNMB

Background:

Background Information: GPNMB is a type I transmembrane glycoprotein which shows homology to the pMEL17 precursor, a melanocyte-specific protein. GPNMB shows expression in the lowly metastatic human melanoma cell lines and xenografts but does not show expression in the highly metastatic cell lines. GPNMB may be involved in growth delay and reduction of metastatic potential.The protein encoded by this gene is a type I transmembrane glycoprotein which shows homology to the pMEL17 precursor, a melanocyte-specific protein. GPNMB shows expression in the lowly metastatic human melanoma cell lines and xenografts but does not show expression in the highly metastatic cell lines. GPNMB may be involved in growth delay and reduction of metastatic potential. Two transcript variants encoding different isoforms have been found for this gene.

Gene Name: Glycoprotein (transmembrane) nmb

Alternative Symbols: NMB, HGFIN, PLCA3

Protein Name: cDNA FLJ78601, highly similar to Homo sapiens glycoprotein (transmembrane) nmb (GPNMB), transcript variant 2, mRNA EMBL BAF84767.1

Molecular Weight: 63 kDa

Gene ID: 10457

NCBI Accession: NP_002501

UniProt: Q14956