BID antibody (pSer61) (Cy5)

Catalog No. ABIN756776

Product Details anti-BID Antibody (Cy5)

Target: BID (BH3 Interacting Domain Death Agonist (BID))

Binding Specificity: pSer61

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This BID antibody is conjugated to Cy5

Application: Western Blotting (WB), Flow Cytometry (FACS), Immunofluorescence (Cultured Cells) (IF (cc)), Immunofluorescence (Paraffin-embedded Sections) (IF (p))

Cross-Reactivity: Human

Predicted Reactivity: Mouse

Purification: Purified by Protein A.

Immunogen: KLH conjugated synthetic phosphopeptide derived from mouse Bid around the phosphorylation site of Ser61

Isotype: IgG

Application Details

Application Notes: FCM 1:20-100
IF(IHC-P) 1:50-200
IF(IHC-F) 1:50-200
IF(ICC) 1:50-200

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 μg/μL

Buffer: Aqueous buffered solution containing 0.01M TBS ( pH 7.4) with 1 % BSA, 0.03 % Proclin300 and 50 % Glycerol.

Preservative: ProClin

Precaution of Use: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Store at -20°C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles.

Expiry Date: 12 months

Target Details for BID

Target: BID (BH3 Interacting Domain Death Agonist (BID))

Alternative Name: Bid (BID Products)

Synonyms: FP497 antibody, 2700049M22Rik antibody, AI875481 antibody, AU022477 antibody, BID antibody, bid antibody, si:ch211-238n5.6 antibody, fp497 antibody, xbid antibody, DKFZp469E066 antibody, BH3 interacting domain death agonist antibody, BH3 interacting domain death agonist S homeolog antibody, BID antibody, Bid antibody, bida antibody, bid.S antibody, bid antibody

Background:

Synonyms: Bid phospho S61, p-Bid phospho S61, BH3 interacting domain death agonist, p22 BID, BID, BH3-interacting domain death agonist, AltName: BH3-interacting domain death agonist p15, BH3-interacting domain death agonist p13, p13 BID, BH3-interacting domain death agonist p11, p11 BID.

Background: Bid, a BH3 domain containing proapoptotic Bcl2 family member, is localized in the cytosolic fraction of cells as an inactive precursor. Its active form is generated upon proteolytic cleavage by caspase 8 in the Fas signaling pathway. Cleaved Bid translocates to mitochondria and releases its potent proapoptotic activity, which in turn induces cytochrome c release and mitochondrial damage. The cytochrome c releasing activity of Bid was antagonized by Bcl2. Mutation in the SH3 domain can diminish the cytochrome c releasing activity. In animal model studies, Bid deficient mice are found resistant to the lethal effects of death factor signals relayed through Fas.

Gene ID: 12122

Pathways: Apoptosis, Caspase Cascade in Apoptosis, Positive Regulation of Endopeptidase Activity