MYCN antibody (Mycn) (AA 410-464)

Details for Product anti-MYCN Antibody No. ABIN760676, Supplier: Log in to see
Antigen
  • MODED
  • MYCNOT
  • N-myc
  • NMYC
  • ODED
  • bHLHe37
  • Nmuc1
  • Nmyc
  • Nmyc-1
  • Nmyc1
  • c-nmyc
  • olfactory receptor, family 8, subfamily S, member 1-like
  • v-myc avian myelocytomatosis viral oncogene neuroblastoma derived homolog
  • v-myc myelocytomatosis viral related oncogene, neuroblastoma derived
  • v-myc myelocytomatosis viral related oncogene, neuroblastoma derived (avian)
  • LOC515887
  • MYCN
  • Mycn
Epitope
AA 410-464
14
8
4
4
4
3
3
2
2
2
1
1
1
Reactivity
Human, Mouse (Murine), Rat (Rattus)
90
55
29
3
2
2
2
2
1
1
1
Host
Rabbit
57
35
Clonality
Polyclonal
Conjugate
This MYCN antibody is un-conjugated
2
2
2
2
2
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
Application
Immunofluorescence (Paraffin-embedded Sections) (IF (p)), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)
69
20
18
17
16
12
4
4
3
3
3
3
2
2
1
1
Options
Supplier
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Available images

'Independent Validation' Badge
Lot Number 120917
Method validated Western Blotting
Positive Control MCF7 cells (variant MCF7/VP)00284-0/pdf)
Negative Control SK-N-SH cells - RNA
Notes A strong band was observed in the positive control sample at the correct molecular weight. An additional band was also observed in the positive sample at a higher molecular weight which may represent non-specific binding. No bands were observed in the negative control sample.
Primary Antibody
  • Antigen: Mycn (MYCN)
  • Catalog number: ABIN760676
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  • Supplier catalog number: Log in to see
  • Lot number: 120917
  • Antibody Dilution: 1:200
Secondary Antibody
  • Antigen: Goat Anti-Rabbit IgG (H + L)-HRP Conjugate
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  • Catalog number: Log in to see
  • Lot number: L170-6515
  • Antibody Dilution: 1:10,000
Controls
  • Positive control: MCF7 cells
  • Negative control: SK-N-SH cells
Protocol
  • 1. The cell extracts were heated at 95°C for 5 minutes in 1X SDS Sample Buffer containing 1% SDS and 1.25% β-mercaptoethanol.
  • 2. 15 μl of heated culture-media were loaded and resolved on 8-16% SDS-polyacrylamide gel.
  • 3. The Thermo Scientific - Spectra Multicolor Broad Range (Cat # 26634) were used as molecular mass markers.
  • 4. Proteins were then transferred onto PVDF membrane by wet transfer and protein transfer was confirmed with Ponceau-S staining.
  • 5. The PVDF membrane was incubated with 25 ml of blocking buffer [Tris Buffered Saline, pH 7.4 plus 0.1% TW20 (TBST)] containing 5% (W/V) BSA at room temperature for 1 hour.
  • 6. The membrane was rinsed with TBST once.
  • 7. The membrane was immersed with the protein side up in the primary antibody solution in TBST containing 5% (W/V) BSA and incubated for 13 hours at 4°C.
  • 8. The membrane was rinsed in TBST thrice for 5 minutes each.
  • 9. The membrane was incubated in the HRP-conjugated secondary antibody solution in TBST containing 5% (W/V) BSA and incubated for 1 hour at room temperature (~26°C) with gentle agitation.
  • 10. The membrane was rinsed thrice TBST thrice for 5 minutes each.
  • 11. The membrane was rinsed in TBS twice for 30 seconds each.
  • 12. Signals were detected with ECL-2 Substrate. The blot was scanned for 45 minutes.
  • 13. The membrane was rinsed three times TBST.
  • 14. Incubated in Acidic Glycine Stripping Buffer at room temperature with gentle agitation for 3 times, 10 minutes each.
  • 15. The membrane was washed in TBST 2 times for 10 minutes each.
  • 16. Repeated Steps 5-12 with the loading control antibody (for Anti-actin) and its matching secondary antibody.
Experimental Notes - No experimental challenges noted.
Validation Images
Western Blotting validation image for anti-Mycn (MYCN) (AA 410-464) antibody (ABIN760676) Figure 1: Western Blot for MYCN. Arrowhead indicates the expected molecular weight of...
Immunogen KLH conjugated synthetic peptide derived from human n-Myc
Isotype IgG
Purification Purified by Protein A.
Alternative Name N-Myc (MYCN Antibody Abstract)
Background

This gene is a member of the MYC family and encodes a protein with a basic helix-loop-helix (bHLH) domain. This protein is located in the nucleus and must dimerize with another bHLH protein in order to bind DNA. Amplification of this gene is associated with a variety of tumors, most notably neuroblastomas. [provided by RefSeq, Jul 2008].

Subcellular location: Nucleus

Synonyms: NMYC, ODED, MODED, N-myc, bHLHe37, N-myc proto-oncogene protein, Class E basic helix-loop-helix protein 37, MYCN

Gene ID 4613
UniProt P04198
Research Area Transcription Factors
Application Notes WB 1:100-1000
IHC-P 1:100-500
IF(IHC-P) 1:50-200
Restrictions For Research Use only
Format Liquid
Concentration 1 μg/μL
Buffer Aqueous buffered solution containing 1 % BSA, 50 % glycerol and 0.09 % sodium azide.
Preservative Sodium azide
Precaution of Use This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.
Storage -20 °C
Storage Comment Store at -20°C
Expiry Date 12 months
Supplier Images
Immunohistochemistry (IHC) image for anti-MYCN antibody (Mycn) (AA 410-464) (ABIN760676) Formalin-fixed and paraffin embedded human colon carcinoma labeled with Anti-n-Myc Po...
Immunohistochemistry (IHC) image for anti-MYCN antibody (Mycn) (AA 410-464) (ABIN760676) Formalin-fixed and paraffin embedded human brain labeled with Anti-n-Myc Polyclonal A...
Western Blotting (WB) image for anti-MYCN antibody (Mycn) (AA 410-464) (ABIN760676) Image provided by the Independent Validation Program (badge number 29800). Lane 1: MC...
Product cited in: Ramraj, Aravindan, Somasundaram, Herman, Natarajan, Aravindan: "Serum-circulating miRNAs predict neuroblastoma progression in mouse model of high-risk metastatic disease." in: Oncotarget, Vol. 7, Issue 14, pp. 18605-19, 2016 (PubMed).