Formalin-fixed and paraffin embedded human colon carcinoma labeled with Anti-n-Myc Polyclonal Antibody, Unconjugated (ABIN760676) at 1:200 followed by conjugation to the secondary antibody and DAB staining
Image provided by the Independent Validation Program (badge number 29800). Lane 1: MCF7 cell lysates, Lane 2: SK-N-SH cell lysates probed with Rabbit Anti-n-Myc Polyclonal Antibody, Unconjugated at 1:200 overnight at 4˚C. Followed by conjugation to secondary antibody at 1:10000 for 60 min at 26˚C.
A strong band was observed in the positive control sample at the correct molecular weight. An additional band was also observed in the positive sample at a higher molecular weight which may represent non-specific binding. No bands were observed in the negative control sample.