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CD14 antibody

CD14 Reactivity: Human FACS Host: Mouse Monoclonal FWKW unconjugated
Catalog No. ABIN793397
  • Target See all CD14 Antibodies
    CD14
    Reactivity
    • 247
    • 47
    • 30
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    Human
    Host
    • 204
    • 64
    • 16
    • 3
    • 1
    Mouse
    Clonality
    • 224
    • 64
    Monoclonal
    Conjugate
    • 121
    • 29
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    • 2
    • 2
    • 2
    • 1
    • 1
    • 1
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    This CD14 antibody is un-conjugated
    Application
    • 202
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    Flow Cytometry (FACS)
    Specificity
    Human CD14
    Purification
    Protein A/G purified
    Immunogen


    Type of Immunogen: Native protein
    Clone
    FWKW
    Isotype
    IgG2b
    Top Product
    Discover our top product CD14 Primary Antibody
  • Application Notes
    Approved: Flo

    Usage: PBMC: Add 10 μL of antibody/10^6 PBMC in 100 μL PBS. Mix gently and incubate for 15 minutes at 2 to 8 °C. Wash twice with PBS and analyze or fix with 0.5 % v/v of paraformaldehyde in PBS and analyze. WHOLE BLOOD: Add 10 μL of antibody/100 μL of whole blood. Mix gently and incubate for 15 minutes at room temperature (20 °C). Lyse the whole blood. Wash once with PBS and analyze or fix with 0.5 % v/v of paraformaldehyde in PBS and analyze. See instrument manufacturers instructions for Lysed Whole Blood and Immunofluorescence analysis with a flow cytometer or microscope. ALLOPHYCOCYANIN: (APC) conjugates are analyzed in multi-color flow cytometry with instruments equipped with a second laser and proper filters. Laser excitation is at 633 nm with a Helium Neon (HeNe) laser or a 600-640 nm (633 nm) range for a Dye laser. Peak fluorescence emission is at 660 nm.
    Comment

    Target Species of Antibody: Human

    Restrictions
    For Research Use only
  • Format
    Liquid
    Concentration
    Lot specific
    Buffer
    PBS, 0.08 % Sodium Azide
    Preservative
    Sodium azide
    Precaution of Use
    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
    Handling Advice
    Aliquot to Avoid freeze/thaw cycles.
    Storage
    -20 °C
    Storage Comment
    Store at -20°C. Aliquot to avoid freeze/thaw cycles.
  • Target
    CD14
    Alternative Name
    CD14 (CD14 Products)
    Synonyms
    CD14 molecule antibody, CD14 antigen antibody, CD14 antibody, Cd14 antibody
    Background
    Name/Gene ID: CD14

    Synonyms: CD14, CD14 Molecule, CD14 antigen
    Gene ID
    929
    UniProt
    P08571
    Pathways
    TLR Signaling, Activation of Innate immune Response, Cellular Response to Molecule of Bacterial Origin, Toll-Like Receptors Cascades
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