ITGAE antibody (Integrin, alpha E (Antigen CD103, Human Mucosal Lymphocyte Antigen 1, alpha Polypeptide))

Details for Product anti-ITGAE Antibody No. ABIN810076, Supplier: Log in to see
Antigen
  • A530055J10
  • CD103
  • aM290
  • alpha-E1
  • alpha-M290
  • HUMINAE
  • integrin alpha E, epithelial-associated
  • integrin, alpha E
  • integrin, alpha E (antigen CD103, human mucosal lymphocyte antigen 1; alpha polypeptide)
  • Itgae
  • ITGAE
Reactivity
Human, Non-Human Primate
217
108
24
23
11
3
2
2
Host
Mouse
190
63
46
19
9
7
1
Clonality (Clone)
Monoclonal ()
Conjugate
This ITGAE antibody is un-conjugated
45
43
23
14
10
10
6
5
5
5
5
5
5
5
5
4
4
3
3
3
2
2
2
2
1
1
1
Application
Flow Cytometry (FACS), Immunohistochemistry (Formalin-fixed Sections) (IHC (f)), Immunoprecipitation (IP), Western Blotting (WB)
250
126
99
91
86
35
19
11
8
7
5
4
4
3
2
2
2
2
2
1
1
Options
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Immunogen HTLV-1 induced human T cell line MAPS16
Clone Ber-ACT8
Isotype IgG1
Specificity The mouse monoclonal antibody Ber-ACT8 recognizes CD103 (alpha E integrin), a type I transmembrane glycoprotein primarily found on intestinal intraepithelial lymphocytes.
HLDA V, WS Code A067
Purification Purified from cell culture supernatant by protein-A affinity chromatography.
Purity > 95 % (by SDS-PAGE)
Alternative Name ITGAE (ITGAE Antibody Abstract)
Background CD103 / Integrin alphaE is an integrin subunit that is expressed on intraepithelial lymphocytes, epithelial dendritic cells, lamina propria-derived dendritic cells, a subpopulation of lamina propria T cells, a small subset of peripheral lymphocytes, namely T reg cells, and on activated and TGF-beta stimulated lymphocytes. CD103 is in mature form cleaved into two disulfide-linked chains (C-terminal 150 kDa chain and N-terminal 25 kDa chain). It heterodimerizes with integrin beta7 subunit to form alphaE/beta7 integrin (mucosal lymphocyte 1 antigen), which through binding E-cadherin mediates homing of lymphocytes to the intestinal epithelium, and, in addition to the role in adhesion, may serve as an accessory molecule for intraepithelial lymphocyte activation.
Gene ID 3682
Research Area Surface Receptors of Immune Cells, CD Antigens
Application Notes Optimal working dilution should be determined by the investigator.
Restrictions For Research Use only
Concentration 1 mg/mL
Buffer Phosphate buffered saline (PBS) with 15 mM sodium azide, approx. pH 7.4
Handling Advice Do not freeze.
Storage 4 °C
Storage Comment Store at 2-8°C. Do not freeze. Do not use after expiration date stamped on vial label.
Product cited in: Zhang, Bertucci, Ramsey-Goldman, Burt, Datta et al.: "Regulatory T cell (Treg) subsets return in patients with refractory lupus following stem cell transplantation, and TGF-beta-producing CD8+ Treg cells are associated with immunological remission of ..." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 183, Issue 10, pp. 6346-58, 2009 (PubMed).

Jaensson, Uronen-Hansson, Pabst, Eksteen, Tian, Coombes, Berg, Davidsson, Powrie, Johansson-Lindbom, Agace: "Small intestinal CD103+ dendritic cells display unique functional properties that are conserved between mice and humans." in: The Journal of experimental medicine, Vol. 205, Issue 9, pp. 2139-49, 2008 (PubMed).

Cardoso, Garlet, Moreira, Júnior, Rossi, Silva: "Characterization of CD4+CD25+ natural regulatory T cells in the inflammatory infiltrate of human chronic periodontitis." in: Journal of leukocyte biology, Vol. 84, Issue 1, pp. 311-8, 2008 (PubMed).

Kost, Holden, Mann: "Marginal zone B-cell lymphoma: a retrospective immunophenotypic analysis." in: Cytometry. Part B, Clinical cytometry, Vol. 74, Issue 5, pp. 282-6, 2008 (PubMed).

Sigmundsdottir, Johnston, Gudjonsson, Bjarnason, Valdimarsson: "Methotrexate markedly reduces the expression of vascular E-selectin, cutaneous lymphocyte-associated antigen and the numbers of mononuclear leucocytes in psoriatic skin." in: Experimental dermatology, Vol. 13, Issue 7, pp. 426-34, 2004 (PubMed).

León, Roy: "Isolation of human small bowel intraepithelial lymphocytes by annexin V-coated magnetic beads." in: Laboratory investigation; a journal of technical methods and pathology, Vol. 84, Issue 6, pp. 804-9, 2004 (PubMed).

Brandes, Willimann, Lang, Nam, Jin, Brenner, Morita, Moser: "Flexible migration program regulates gamma delta T-cell involvement in humoral immunity." in: Blood, Vol. 102, Issue 10, pp. 3693-701, 2003 (PubMed).

Poles, Barsoum, Yu, Yu, Sun, Daly, He, Mehandru, Talal, Markowitz, Hurley, Ho, Zhang: "Human immunodeficiency virus type 1 induces persistent changes in mucosal and blood gammadelta T cells despite suppressive therapy." in: Journal of virology, Vol. 77, Issue 19, pp. 10456-67, 2003 (PubMed).

French, Cresswell, Wong, Seymour, Charnley, Kirby: "T cell adhesion and cytolysis of pancreatic cancer cells: a role for E-cadherin in immunotherapy?" in: British journal of cancer, Vol. 87, Issue 9, pp. 1034-41, 2002 (PubMed).