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SH3 and Multiple Ankyrin Repeat Domains 3 (SHANK3) (AA 840-857) antibody

Details for Product No. ABIN863129, Supplier: Log in to see
Antigen
  • DEL22q13.3
  • PROSAP2
  • PSAP2
  • SCZD15
  • SPANK-2
  • Prosap2
  • AI841104
  • Shank3b
Epitope
AA 840-857
36
19
5
2
2
1
1
1
1
Reactivity
Human, Mouse (Murine), Rat (Rattus)
75
61
46
2
Host
Mouse
59
27
1
Clonality (Clone)
Monoclonal ()
Conjugate
Un-conjugated
2
2
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
Application
Immunocytochemistry (ICC), Immunofluorescence (IF), Immunoprecipitation (IP), Immunohistochemistry (IHC), Western Blotting (WB)
76
66
55
40
38
11
4
4
2
1
1
Supplier
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Immunogen Synthetic peptide amino acids 840-857 of rat Shank3
Clone S69-46
Specificity Detects ~190 kDa. No cross-reactivity against Shank1 or Shank2.
Sensitivity 1 µg/mL of SMC-336 was sufficient for detection of Shank3 in 10 µg COS cell lysate transiently transfected with Shank3 by colorimetric immunoblot analysis using goat anti-mouse IgG:HRP as the secondary antibody.
Purification Protein G Purified
Alternative Name SHANK3 (SHANK3 Antibody Abstract)
Background Shank proteins make up a family of scaffold proteins identified through their interaction with a variety of membrane and cytoplasmic proteins (1). Shank proteins at postsynaptic sites of excitatory synapses play roles in signal transmission into the postsynaptic neuron. Shank proteins are also crucial in receptor tyrosine kinase signaling, specifically, Shank3 can mediate Erk-MAPK and P13K signaling which is crucial for tubule formation (2). Shank3 is also one of the latest genes to be associated with autism. A mutation of a single copy of Shank3 on chromosome 22q13 can result in language and/or social communication disorders (3).
Cellular Localization: Cytoplasm | Cell Junction | Synapse | Postsynaptic Cell Membrane | Postsynaptic Density
Gene ID 59312
NCBI Accession NP_067708
UniProt Q9JLU4
Research Area Neurology
Application Notes Recommended Dilution: WB (1:1000), IHC (1:100), ICC/IF (1:100), optimal dilutions for assays should be determined by the user.
Restrictions For Research Use only
Format Liquid
Concentration 1 mg/mL
Buffer PBS pH 7.4, 50 % glycerol, 0.09 % sodium azide
Preservative Sodium azide
Precaution of Use This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage -20 °C
Supplier Images
Western Blotting (WB) image for anti-SH3 and Multiple Ankyrin Repeat Domains 3 (SHANK3) (AA 840-857) antibody (ABIN863129) Shank3 Brain Membrane Western Blotting.
Background publications Durand, Betancur, Boeckers et al.: "Mutations in the gene encoding the synaptic scaffolding protein SHANK3 are associated with autism spectrum disorders." in: Nature genetics, Vol. 39, Issue 1, pp. 25-7, 2006 (PubMed).