E-cadherin antibody

Catalog No. ABIN865509

The Mouse Monoclonal anti-E-cadherin antibody (Clone 7H12) (ABIN865509) specifically detects E-cadherin in WB, IHC, FACS and ELISA. The antibody is reactive with Human, Mouse and Monkey samples.

Quick Overview for E-cadherin antibody (ABIN865509)

Target: E-cadherin (CDH1) (Cadherin 1, Type 1, E-Cadherin (Epithelial) (CDH1))

Reactivity: Human, Mouse, Monkey

Host: Mouse

Clonality: Monoclonal

Conjugate: This E-cadherin antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Flow Cytometry (FACS), ELISA

Clone: 7H12

Product Details anti-E-cadherin Antibody

Purification: Ascitic fluid

Immunogen: Purified recombinant fragment of human CDH1 expressed in E. Coli.

Isotype: IgG1

Application Details

Application Notes: WB: 1/500 - 1/2000, IHC: 1/200 - 1/1000, FC: 1/200-1/400, ELISA: Propose dilution 1/10000.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 100g/100l

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C

Target Details for E-cadherin

Target: E-cadherin (CDH1) (Cadherin 1, Type 1, E-Cadherin (Epithelial) (CDH1))

Alternative Name: CDH1

Background: E-Cadherin is a 120 kDa transmembrane glycoprotein that is localized in the adherens junctions of epithelial cells. There, it interacts with the cytoskeleton through the associated cytoplasmic catenin proteins. In addition to being a calcium-dependent adhesion molecule, E-Cadherin is also a critical regulator of epithelial junction formation. Its association with catenins is necessary for cell-cell adhesion. These E-cadherin/catenin complexes associate with corical actin bundles at both the zonula adherens and the lateral adhesion plaques. Tyrosine phosphorylation can disrupt these complexes, leading to changes in cell adhesion properties. E-Cadherin expression is often down-regulated in highly invasive, poorly differentiated carcinomas. Increased expression of E-Cadherin in these cells reduces invasiveness. Thus, loss of expression or function of E-Cadherin appears to be an important step in tumorigenic progression. Tissue specificity: Non-neural epithelial tissues. Synonyms: UVO, CDHE, ECAD, LCAM, Arc-1, CD324, CDH1

Molecular Weight: 135kDa

Gene ID: 999

Pathways: WNT Signaling, Sensory Perception of Sound, Cell-Cell Junction Organization, Tube Formation