FAS antibody (FITC)

Catalog No. ABIN920646

Product Details anti-FAS Antibody (FITC)

Target: FAS (TNF Receptor Superfamily, Member 6 (FAS))

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This FAS antibody is conjugated to FITC

Application: Flow Cytometry (FACS), Immunohistochemistry (IHC)

Specificity: Reacts specifically with the human Fas antigen, although the epitope has not been mapped. Binds both the soluble and membrane-bound forms of Fas. Does not react with mouse or gibbon Fas. Recognizes activated T-cells and a subpopulation of B-cells. The cell-surface Fas antigen is a 48 kDa transmembrane protein that can mediate apoptosis/programmed cell death and belongs to the nerve growth factor (NGF)/tumor necrosis factor (TNF) receptor superfamily. The human Fas antigen is expressed in various human cells, including activated peripheral T and B lymphocytes, Various tumor cell lines, leukemic T cells, and myeloid cells. It is also differentially expressed on human thymocytes during thymic maturation.

Characteristics: Mouse myeloma (P3.X63.Ag8.653) x immunized mouse (Balb/c) spleen cells.

Purification: Purified by protein A chromatography

Purity: > 95 % by SDS-PAGE.

Immunogen: Human B lymphoblast cell line SKW6.4 cell surface molecules

Clone: APO-1-1

Isotype: IgG1

Application Details

Application Notes: Immunohistochemistry on cryostat sections and on cytospin preparations. Not suitable for use on paraffin sections. Flow Cytometry: Use at 1:20 dilution (as determined on SKW6.4 cells.

Restrictions: For Research Use only

Handling

Buffer: FITC-labeled antibody in 1 ml PBS, pH 7.4 with 1% BSA and 0.02% NaN3.

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C

Target Details for FAS

Target: FAS (TNF Receptor Superfamily, Member 6 (FAS))

Alternative Name: Fas (FAS Products)

Synonyms: ALPS1A antibody, APO-1 antibody, APT1 antibody, CD95 antibody, FAS1 antibody, FASTM antibody, TNFRSF6 antibody, AI196731 antibody, APO1 antibody, TNFR6 antibody, Tnfrsf6 antibody, lpr antibody, Fas cell surface death receptor antibody, Fas (TNF receptor superfamily member 6) antibody, FAS antibody, Fas antibody, fas antibody

Background: Monoclonal antibody recognizing Fas protein, FITC-labeled for simplified flow cytometry procedures. Clone Apo-1-1 is an IgG1 isotype switch variant of the IgG3 clone APO-1-3 (Cat. No. MC-095).

Pathways: p53 Signaling, Apoptosis, Production of Molecular Mediator of Immune Response, Positive Regulation of Endopeptidase Activity