Caveolin-1 is a major structural component of caveolae which are vasicular invaginations present on the plasma membrane of different cell types. It plays a regulatory role in several signalling pathways and is reported to be most abundantly expressed in terminally differentiated mesenchymal cells such as smooth muscle cells, adipocytes, endothelial cells and fibroblasts.
Characteristics
Positive Control: Immunohistochemistry: Colon. Western blot: MG-63 cell line. Cellular Localization: Cytoplasmic and membrane.
Immunogen
Prokaryotic recombinant protein containing part of the N-terminus domain of the human Caveolin-1 molecule.
Immunohistochemistry: Paraffin sections. Use at a 1:200 dilution. Use a high- temperature antigen unmasking technique with 60-minute primary antibody incubation at 25°C and standard ABC technique. Western blot: Use at ~ 1:4,000 dilution.
Restrictions
For Research Use only
Format
Liquid
Buffer
1 mL of liquid tissue culture supernatant containing monoclonal antibody with 15 mM sodium azide.
Preservative
Sodium azide
Precaution of Use
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.