ELISA: Use at 1.0 µg/mL - 1.0 µg/30 mL. Western blot: Use at 1.0 µg/mL - 1.0 µg/10 mL. Dot blot: Use at 1.0 µg/mL - 1.0 µg/10 mL.
Restrictions
For Research Use only
Format
Liquid
Buffer
1.0 mg/mL in TBS pH 7.2 with 0.1% sodium azide.
Preservative
Sodium azide
Precaution of Use
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage
4 °C
Target
GFP
(Green Fluorescent Protein (GFP))
Alternative Name
GFP (Green Fluorescent Protein)
Background
Rabbits were immunized with the highly purified green fluorescent protein (GFP) isolated directly from Aequorea victoria. After multiple immunizations in Freund's adjuvant, serum was collected. Antibodies were immunoaffinity- purified using native GFP immobilized on a solid phase.