RASGRF1 antibody (Middle Region)

Catalog No. ABIN928864

This Rabbit Polyclonal antibody specifically detects RASGRF1 in WB. It exhibits reactivity toward Human.

Quick Overview for RASGRF1 antibody (Middle Region) (ABIN928864)

Target: RASGRF1 (Ras Protein-Specific Guanine Nucleotide-Releasing Factor 1 (RASGRF1))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This RASGRF1 antibody is un-conjugated

Application: Western Blotting (WB)

Product Details anti-RASGRF1 Antibody

Binding Specificity: Middle Region

Purification: Purified

Immunogen: RASGRF1 antibody was raised in rabbit using the middle region of RASGRF1 as the immunogen

Application Details

Application Notes: WB: 0.2-1 µg/mL
Optimal conditions should be determined by the investigator.

Comment:

RASGRF1 Blocking Peptide, (ABIN5615766), is also available for use as a blocking control in assays to test for specificity of this RASGRF1 antibody

Restrictions: For Research Use only

Handling

Format: Lyophilized

Concentration: Lot specific

Buffer: Lyophilized powder. Add 50 µL of distilled water. Final antibody concentration is 1 mg/mL in PBS buffer.

Handling Advice: Avoid repeated freeze/thaw cycles.

Storage: 4 °C/-20 °C

Storage Comment: Store at 4 °C, following reconstitution, aliquot and store at -20 °C.

Target Details for RASGRF1

Target: RASGRF1 (Ras Protein-Specific Guanine Nucleotide-Releasing Factor 1 (RASGRF1))

Alternative Name: RASGRF1

Background: The protein encoded by this gene is a guanine nucleotide exchange factor (GEF) similar to the Saccharomyces cerevisiae CDC25 gene product. Functional analysis has demonstrated that this protein stimulates the dissociation of GDP from RAS protein. The studies of the similar gene in mouse suggested that the Ras-GEF activity of this protein in brain can be activated by Ca2+ influx, muscarinic receptors, and G protein beta-gamma subunit. Mouse studies also indicated that the Ras-GEF signaling pathway mediated by this protein may be important for long-term memory. Alternatively spliced transcript variants encoding distinct isoforms have been reported. Synonyms: Polyclonal RASGRF1 antibody, Anti-RASGRF1 antibody, Ras protein-specific guanine nucleotide-releasing factor 1 antibody, CDC25 antibody, CDC25L antibody, GNRP antibody, GRF1 antibody, GRF55 antibody, H-GRF55 antibody, PP13187 antibody.

Pathways: RTK Signaling, Cell Division Cycle, M Phase