RASGRF1 antibody (N-Term)

Catalog No. ABIN2786747

The Rabbit Polyclonal anti-RASGRF1 antibody is suitable to detect RASGRF1 in samples from Mouse, Human, Rat, Cow, Dog, Guinea Pig, Horse, Rabbit and Zebrafish (Danio rerio). It has been validated for WB.

Quick Overview for RASGRF1 antibody (N-Term) (ABIN2786747)

Target: RASGRF1 (Ras Protein-Specific Guanine Nucleotide-Releasing Factor 1 (RASGRF1))

Reactivity: Mouse, Human, Rat, Cow, Dog, Guinea Pig, Horse, Rabbit, Zebrafish (Danio rerio)

Host: Rabbit

Clonality: Polyclonal

Conjugate: This RASGRF1 antibody is un-conjugated

Application: Western Blotting (WB)

Product Details anti-RASGRF1 Antibody

Binding Specificity: N-Term

Purpose: RASGRF1 Antibody - N-terminal region

Sequence: RELDNNRSAL SAASAFAIAT AGANEGTPNK EKYRRMSLAS AGFPPDQRNG

Specificity: Predicted to recognized both human RASGRF1 isoforms (145, 55kD)

Predicted Reactivity: Cow: 100%, Dog: 100%, Guinea Pig: 100%, Horse: 93%, Human: 100%, Mouse: 85%, Rabbit: 93%, Rat: 100%, Zebrafish: 93%

Characteristics: This is a rabbit polyclonal antibody against RASGRF1. It was validated on Western Blot using a cell lysate as a positive control.

Purification: Affinity Purified

Immunogen: The immunogen is a synthetic peptide directed towards the N terminal region of human RASGRF1

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Comment:

We also sell a specific blocking peptide that can be used in combination with this antibody. You can find the blocking peptide under AAP56516-100UG

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.5 mg/mL

Buffer: Purified antibody supplied in 1x PBS buffer with 0.09 % (w/v) sodium azide and 2 % sucrose.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: prevent freeze-thaw cycles

Storage: 4 °C,-20 °C

Storage Comment: For short term use, store at 2-8C up to 1 week. For long term storage, store at -20 °C in small aliquots to prevent freeze-thaw cycles.

Target Details for RASGRF1

Target: RASGRF1 (Ras Protein-Specific Guanine Nucleotide-Releasing Factor 1 (RASGRF1))

Alternative Name: RASGRF1

Background:

Background Information: The protein encoded by this gene is a guanine nucleotide exchange factor (GEF) similar to the Saccharomyces cerevisiae CDC25 gene product. Functional analysis has demonstrated that this protein stimulates the dissociation of GDP from RAS protein.

Gene Name: Ras protein-specific guanine nucleotide-releasing factor 1

Alternative Symbols: GNRP, GRF1, CDC25, GRF55, CDC25L, H-GRF55, PP13187, ras-GRF1

Protein Name: Ras-specific guanine nucleotide-releasing factor 1

Molecular Weight: 54kDa

Gene ID: 5923

NCBI Accession: NP_722522

UniProt: Q13972

Pathways: RTK Signaling, Cell Division Cycle, M Phase