V5 Epitope Tag antibody

Details for Product No. ABIN933572, Supplier: Log in to see
Antigen
Reactivity
Tag
403
18
16
8
3
3
Host
Mouse
248
144
37
7
5
4
3
1
1
Clonality (Clone)
Monoclonal ()
Conjugate
Un-conjugated
31
28
21
14
13
12
12
12
11
10
10
10
10
9
8
8
7
6
5
3
3
3
3
3
2
2
2
1
1
1
1
1
1
1
1
1
1
1
Application
Dot Blot (DB), Immunoprecipitation (IP), ELISA, Western Blotting (WB)
369
263
137
94
78
71
53
44
28
13
9
8
4
4
4
2
2
2
2
1
1
1
Options
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Immunogen V5 Tag antibody was raised in mouse using GKPIPNPLLGLDST (V5) synthetic peptide conjugated to KLH as the immunogen.
Sequence GKPIPNPLLG LDST
Clone E10
Isotype IgG1
Purification Protein A affinity chromatography
Alternative Name V5 Tag
Target Type Tag
Background The V5 epitope tag is derived from a small epitope (Pk) present on the P and V proteins of the paramyxovirus of simian virus 5 (SV5). The V5 tag is usually used with all 14 amino acids (GKPIPNPLLGLDST), although it has also been used with a shorter 9 amino acid sequence (IPNPLLGLD). This tag is particularly useful for western blotting and immunoprecipitation experiments and may also find use in antibody purification.    . Synonyms: Monoclonal V5 Tag antibody, anti-V5 Tag antibody, E10 antibody, V4RR antibody, V5 antibody.
Research Area Tags/Labels
Application Notes WB: 1:1000-3000
Optimal conditions should be determined by the investigator.
Restrictions For Research Use only
Format Liquid
Concentration Lot specific
Buffer Supplied as a liquid in 10 mM PBS, pH 7.2, with 0.05 % NaN3
Preservative Sodium azide
Precaution of Use This product contains Sodium Azide: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.
Handling Advice Avoid repeated freeze/thaw cycles.
Dilute only prior to immediate use.
Storage RT/-20 °C
Storage Comment Store at 4 °C for several days to weeks. Add 0.1 % NaN3 and store at -20 °C for longer periods.
Supplier Images
Western Blotting (WB) image for anti-V5 Epitope Tag antibody (ABIN933572) 1:000 (1 ug/mL) Ab dilution probed against HEK293 cells transfected with V5-tagged pr...
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