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alpha Tubulin antibody (TUBA1) (N-Term)

Details for Product anti-TUBA1 Antibody No. ABIN93891, Supplier: Log in to see
Antigen
  • K-ALPHA-1
  • TUBA2
  • mec-12
  • tuba4
  • alpha tubulin
  • similar to alpha tubulin
  • tubulin-alpha
  • tubulin, alpha 1b
  • tubulin, alpha 3e
  • tubulin, alpha 4b
  • LOC100009327
  • RGD1560000
  • LOC100101229
  • TUBA1B
  • TUBA3E
  • tuba4b
Alternatives
anti-All Species alpha Tubulin antibody for Immunocytochemistry
Epitope
N-Term
18
12
8
6
4
4
4
4
3
2
2
2
2
1
1
1
1
1
1
1
1
Reactivity
All Species
189
114
102
52
49
46
39
28
27
27
26
26
24
24
23
19
19
19
19
15
15
6
5
5
4
3
3
3
3
3
3
2
2
2
2
2
2
2
1
1
1
1
1
Host
Mouse
128
66
16
5
2
2
Clonality (Clone)
Monoclonal ()
Conjugate
This alpha Tubulin antibody is un-conjugated
11
8
8
3
3
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
1
1
1
1
1
1
1
1
1
1
1
1
1
Application
Immunocytochemistry (ICC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)
194
79
72
70
67
43
42
35
23
9
8
4
2
2
2
2
2
1
Options
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Immunogen Fraction of tubulin purified from porcine brain by two cycles of polymerization - depolymerization.
Clone TU-01
Isotype IgG1
Specificity The antibody TU-01 recognizes the defined epitope (aa 65-97) on N-terminal structural domain of alpha-tubulin.
Purification Purified from ascites by precipitation methods.
Purity > 95 % (by SDS-PAGE)
Alternative Name alpha-tubulin (TUBA1 Antibody Abstract)
Background The microtubules are intracellular dynamic polymers made up of evolutionarily conserved polymorphic alpha/beta-tubulin heterodimers and a large number of microtubule-associated proteins (MAPs). The microtubules consist of 13 protofilaments and have an outer diameter 25 nm. Microtubules have their intrinsic polarity, highly dynamic plus ends and less dynamic minus ends. Microtubules are required for vital processes in eukaryotic cells including mitosis, meiosis, maintenance of cell shape and intracellular transport. Microtubules are also necessary for movement of cells by means of flagella and cilia. In mammalian tissue culture cells microtubules have their minus ends anchored in microtubule organizing centers (MTOCs).The GTP (guanosintriphosphate) molecule is an essential for tubulin heterodimer to associate with other heterodimers to form microtubule. In vivo, microtubule dynamics vary considerably. Microtubule polymerization is reversible and a populations of microtubules in cells are on their minus ends either growing or shortening –, this phenomenon is called dynamic instability of microtubules. On a practical level, microtubules can easily be stabilized by the addition of non-hydrolysable analogues of GTP (eg. GMPPCP) or more commonly by anti-cancer drugs such as Taxol. Taxol stabilizes microtubules at room temperature for many hours. Using limited proteolysis by enzymes both tubulin subunits can be divided into N-terminal and C-terminal structural domains. The alpha-tubulin (relative molecular weight around 50 kDa) is globular protein that exists in cells as part of soluble alpha/beta-tubulin dimer or it is polymerized into microtubules. In different species it is coded by multiple tubulin genes that form tubulin classes (in human 6 genes). Expressed tubulin genes are named tubulin isotypes. Some of the tubulin isotypes are expressed ubiquitously, while some have more restricted tissue expression.Alpha-tubulin is also subject of numerous post-translational modifications. Tubulin isotypes and their posttranslational modifications are responsible for multiple tubulin charge variants - tubulin isoforms. Heterogeneity of alpha-tubulin is concentrated in C-terminal structural domain.
Research Area Cytoskeleton
Pathways Microtubule Dynamics
Application Notes Optimal working dilution should be determined by the investigator.
Restrictions For Research Use only
Concentration 1 mg/mL
Buffer Phosphate buffered saline (PBS) with 15 mM sodium azide, approx. pH 7.4
Handling Advice Do not freeze.
Storage 4 °C
Storage Comment Store at 2-8°C. Do not freeze. Do not use after expiration date stamped on vial label.
Supplier Images
Immunofluorescence (IF) image for anti-alpha Tubulin antibody (TUBA1) (N-Term) (ABIN93891) Immunofluorescence staining of 3T3 mouse embryonal fibroblast cell line using anti-al...
Immunofluorescence (IF) image for anti-alpha Tubulin antibody (TUBA1) (N-Term) (ABIN93891) Immunofluorescence staining of HeLa human cervix carcinoma cell line using anti-alpha...
Immunofluorescence (IF) image for anti-alpha Tubulin antibody (TUBA1) (N-Term) (ABIN93891) Nucleus is stained with DAPI (blue).
Western Blotting (WB) image for anti-alpha Tubulin antibody (TUBA1) (N-Term) (ABIN93891) Use of anti-alpha-tubulin antibody as a loading control (A) in an Western blotting ex...
Western Blotting (WB) image for anti-alpha Tubulin antibody (TUBA1) (N-Term) (ABIN93891) Use of anti-alpha-tubulin antibody as a loading control (A) in an Western blotting ex...
Product cited in: Lueck, Hennig, Lommatzsch, Pauleikhoff, Wasmuth: "Complement and UV-Irradiated Photoreceptor Outer Segments Increase the Cytokine Secretion by Retinal Pigment Epithelial Cells." in: Investigative ophthalmology & visual science, Vol. 53, Issue 3, pp. 1406-13, 2012 (PubMed).

Dolezalova, Mraz, Barta, Plevova, Vinarsky, Holubcova, Jaros, Dvorak, Pospisilova, Hampl: "MicroRNAs regulate p21(Waf1/Cip1) protein expression and the DNA damage response in human embryonic stem cells." in: Stem cells (Dayton, Ohio), Vol. 30, Issue 7, pp. 1362-72, 2012 (PubMed).

Pospichalova, Tureckova, Fafilek, Vojtechova, Krausova, Lukas, Sloncova, Takacova, Divoky, Leprince, Plachy, Korinek: "Generation of two modified mouse alleles of the Hic1 tumor suppressor gene." in: Genesis (New York, N.Y. : 2000), 2011 (PubMed).

Cancer: Krupkova, Loja, Redova, Neradil, Zitterbart, Sterba, Veselska: "Analysis of nuclear nestin localization in cell lines derived from neurogenic tumors." in: Tumour biology, 2011 (PubMed). Method employed by authors: Immunohistochemistry (IHC) (Sample species: Human).

Sana, Zambo, Skoda, Neradil, Chlapek, Hermanova, Mudry, Vasikova, Zitterbart, Hampl, Sterba, Veselska: "CD133 expression and identification of CD133/nestin positive cells in rhabdomyosarcomas and rhabdomyosarcoma cell lines." in: Analytical cellular pathology (Amsterdam), 2011 (PubMed).

Hrdinka, Dráber, Stepánek, Ormsby, Otáhal, Angelisová, Brdicka, Paces, Horejsí, Drbal: "PRR7 is a transmembrane adaptor protein expressed in activated T cells involved in regulation of T cell receptor signaling and apoptosis." in: The Journal of biological chemistry, Vol. 286, Issue 22, pp. 19617-29, 2011 (PubMed).

Buarta, Vinarskuy, Holubcovua, Dole?alovua, Verner, Pospui?ilovua, Dvo?uak, Hampl: "Human Embryonic Stem Cells are Capable of Executing G1/S Checkpoint Activation." in: Stem cells (Dayton, Ohio), 2010 (PubMed).

Lukas, Mazna, Valenta, Doubravska, Pospichalova, Vojtechova, Fafilek, Ivanek, Plachy, Novak, Korinek: "Dazap2 modulates transcription driven by the Wnt effector TCF-4." in: Nucleic acids research, Vol. 37, Issue 9, pp. 3007-20, 2009 (PubMed).

Kukharskyy, Sulimenko, Mac?rek, Sulimenko, Dráberová, Dráber: "Complexes of gamma-tubulin with nonreceptor protein tyrosine kinases Src and Fyn in differentiating P19 embryonal carcinoma cells." in: Experimental cell research, Vol. 298, Issue 1, pp. 218-28, 2004 (PubMed).

Smertenko, Blume, Viklický, Opatrný, Dráber: "Post-translational modifications and multiple tubulin isoforms in Nicotiana tabacum L. cells." in: Planta, Vol. 201, Issue 3, pp. 349-58, 1997 (PubMed).

Smertenko, Blume, Viklický, Dráber: "Exposure of tubulin structural domains in Nicotiana tabacum microtubules probed by monoclonal antibodies." in: European journal of cell biology, Vol. 72, Issue 2, pp. 104-12, 1997 (PubMed).

Nováková, Dráberová, Schürmann, Czihak, Viklický, Dr-aber: "gamma-Tubulin redistribution in taxol-treated mitotic cells probed by monoclonal antibodies." in: Cell motility and the cytoskeleton, Vol. 33, Issue 1, pp. 38-51, 1996 (PubMed).

Linhartová, Dráber, Dráberová, Viklický: "Immunological discrimination of beta-tubulin isoforms in developing mouse brain. Post-translational modification of non-class-III beta-tubulins." in: The Biochemical journal, Vol. 288 ( Pt 3), pp. 919-24, 1993 (PubMed).

Dráber, Dráberová, Viklický: "Immunostaining of human spermatozoa with tubulin domain-specific monoclonal antibodies. Recognition of a unique beta-tubulin epitope in the sperm head." in: Histochemistry, Vol. 95, Issue 5, pp. 519-24, 1991 (PubMed).

Dráber, Dráberová, Linhartová, Viklický: "Differences in the exposure of C- and N-terminal tubulin domains in cytoplasmic microtubules detected with domain-specific monoclonal antibodies." in: Journal of cell science, Vol. 92 ( Pt 3), pp. 519-28, 1990 (PubMed).

Dráber, Dráberová, Zicconi, Sellitto, Viklický, Cappuccinelli: "Heterogeneity of microtubules recognized by monoclonal antibodies to alpha-tubulin." in: European journal of cell biology, Vol. 41, Issue 1, pp. 82-8, 1987 (PubMed).

Background publications Miyajima, Maruyama, Nonomura, Hatakeyama: "TRIM36 interacts with the kinetochore protein CENP-H and delays cell cycle progression." in: Biochemical and biophysical research communications, Vol. 381, Issue 3, pp. 383-7, 2009 (PubMed).

Honys, R?nuak, Fecikovua, Jedelskuy, Nebesuarovua, Dobrev, Capkovua: "Cytoskeleton-associated large RNP complexes in tobacco male gametophyte (EPPs) are associated with ribosomes and are involved in protein synthesis, processing, and localization." in: Journal of proteome research, Vol. 8, Issue 4, pp. 2015-31, 2009 (PubMed).

Bon, Di Carlo, Folgiero, Avetrani, Lazzari, DOrazi, Brizzi, Sacchi, Soddu, Blandino, Mottolese, Falcioni: "Negative regulation of beta4 integrin transcription by homeodomain-interacting protein kinase 2 and p53 impairs tumor progression." in: Cancer research, Vol. 69, Issue 14, pp. 5978-86, 2009 (PubMed).

Tsao, Tsai, Tung, Chen, Yue, Liao, Wang, Jiang: "Function of CSE1L/CAS in the secretion of HT-29 human colorectal cells and its expression in human colon." in: Molecular and cellular biochemistry, Vol. 327, Issue 1-2, pp. 163-70, 2009 (PubMed).