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CD19 antibody (CD19 Molecule)

Details for Product anti-CD19 Antibody No. ABIN94013, Supplier: Log in to see
Antigen
  • CD19
  • AW495831
  • B4
  • CVID3
  • spinster homolog 1 (Drosophila)
  • CD19 molecule
  • CD19 antigen
  • SPNS1
  • CD19
  • Cd19
Alternatives
anti-Human CD19 antibody for Immunoprecipitation
Reactivity
Human
1012
383
47
16
16
4
3
3
Host
Mouse
851
244
234
12
1
Clonality (Clone)
Monoclonal ()
Conjugate
This CD19 antibody is un-conjugated
133
125
96
67
37
28
26
24
23
23
23
22
17
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16
16
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8
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4
4
4
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2
2
2
1
1
1
1
1
1
1
1
1
1
1
1
1
1
Application
Flow Cytometry (FACS), Immunoprecipitation (IP)
976
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235
176
128
124
89
75
66
53
23
19
18
15
9
7
5
5
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4
4
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4
4
3
2
2
1
1
1
Options
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Immunogen Daudi human Burkitt lymphoma cell line
Clone LT19
Isotype IgG1
Specificity The antibody LT19 reacts with CD19 (B4), a 95 kDa type I transmembrane glycoprotein (immunoglobulin superfamily) expressed on B lymphocytes and follicular dendritic cells, it is lost on plasma cells.
HLDA 10
Purification Purified from cell culture supernatant by protein-A affinity chromatography.
Purity > 95 % (by SDS-PAGE)
Alternative Name CD19 (CD19 Antibody Abstract)
Background CD19 is a transmembrane glycoprotein of Ig superfamily expressed by B cells from the time of heavy chain rearrangement until plasma cell differentiation. It forms a tetrameric complex with CD21 (complement receptor type 2), CD81 (TAPA-1) and Leu13. Together with BCR (B cell antigen receptor), this complex signals to decrease B cell treshold for activation by the antigen. Besides being signal-amplifying coreceptor for BCR, CD19 can also signal independently of BCR coligation and it turns out to be a central regulatory component upon which multiple signaling pathways converge. Mutation of the CD19 gene results in hypogammaglobulinemia, whereas CD19 overexpression causes B cell hyperactivity.
Research Area Stem Cells, Hematopoietic Progenitors, Hematopoietic Stem Cells, Adaptive Immunity, CD Antigens, Surface Receptors of Immune Cells
Pathways Fc-epsilon Receptor Signaling Pathway, EGFR Signaling Pathway, Neurotrophin Signaling Pathway
Application Notes Optimal working dilution should be determined by the investigator.
Restrictions For Research Use only
Concentration 1 mg/mL
Buffer Phosphate buffered saline with 15 mM sodium azide, pH 7.4
Handling Advice Do not freeze.
Storage 4 °C
Storage Comment Store at 2-8°C. Do not freeze. Do not use after expiration date stamped on vial label.
Supplier Images
Flow Cytometry (FACS) image for anti-CD19 antibody (CD19 Molecule) (ABIN94013) Surface staining of human peripheral blood cells with anti-human CD19 (LT19) APC.
Product cited in: Kayserova, Vcelakova, Stechova, Dudkova, Hromadkova, Sumnik, Kolouskova, Spisek, Sediva: "Decreased dendritic cell numbers but increased TLR9-mediated interferon-alpha production in first degree relatives of type 1 diabetes patients." in: Clinical immunology (Orlando, Fla.), Vol. 153, Issue 1, pp. 49-55, 2014 (PubMed).

Background publications Svensson: "Isolation and culture of human hematopoietic progenitors for studies of dendritic cell biology." in: Methods in molecular biology (Clifton, N.J.), Vol. 531, pp. 187-202, 2009 (PubMed).

Gonçalves, Castro, Henriques, Oliveira, Pinheiro, Oliveira, Sreenu, Evans, Davis, Moreira, Carmo: "Molecular cloning and analysis of SSc5D, a new member of the scavenger receptor cysteine-rich superfamily." in: Molecular immunology, Vol. 46, Issue 13, pp. 2585-96, 2009 (PubMed).

Rayment, Kooij, Zhang, Siebold, Murphy, Allen, Willcox, Roberts: "Evidence for the specificity for platelet HPA-1a alloepitope and the presenting HLA-DR52a of diverse antigen-specific helper T cell clones from alloimmunized mothers." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 183, Issue 1, pp. 677-86, 2009 (PubMed).

Shao, Suresh, Vakil, Gomer, Pilling: "Pivotal Advance: Th-1 cytokines inhibit, and Th-2 cytokines promote fibrocyte differentiation." in: Journal of leukocyte biology, Vol. 83, Issue 6, pp. 1323-33, 2008 (PubMed).

Allen, Pang, Skowera, Ellis, Rackham, Lozanoska-Ochser, Tree, Leslie, Tremble, Dayan, Peakman: "Plasmacytoid dendritic cells are proportionally expanded at diagnosis of type 1 diabetes and enhance islet autoantigen presentation to T-cells through immune complex capture." in: Diabetes, Vol. 58, Issue 1, pp. 138-45, 2008 (PubMed).

Barat, Gilbert, Imbeault, Tremblay: "Extracellular ATP reduces HIV-1 transfer from immature dendritic cells to CD4+ T lymphocytes." in: Retrovirology, Vol. 5, pp. 30, 2008 (PubMed).

Harrison, Franklin, Campbell: "Enumeration of blood dendritic cells in patients with multiple myeloma at presentation and through therapy." in: Leukemia & lymphoma, Vol. 49, Issue 12, pp. 2272-83, 2008 (PubMed).

Vantourout, Martinez, Fabre, Collet, Champagne: "Ecto-F1-ATPase and MHC-class I close association on cell membranes." in: Molecular immunology, Vol. 45, Issue 2, pp. 485-92, 2007 (PubMed).

Shi, Xie, Chang, Zhou, Tedder, Mohan: "CD19 hyperexpression augments Sle1-induced humoral autoimmunity but not clinical nephritis." in: Arthritis and rheumatism, Vol. 56, Issue 9, pp. 3057-69, 2007 (PubMed).

Inabe, Kurosaki: "Tyrosine phosphorylation of B-cell adaptor for phosphoinositide 3-kinase is required for Akt activation in response to CD19 engagement." in: Blood, Vol. 99, Issue 2, pp. 584-9, 2002 (PubMed).

Fujimoto, Poe, Jansen, Sato, Tedder: "CD19 amplifies B lymphocyte signal transduction by regulating Src-family protein tyrosine kinase activation." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 162, Issue 12, pp. 7088-94, 1999 (PubMed).