CD19 antibody (CD19 Molecule) (PE)

Details for Product anti-CD19 Antibody No. ABIN94016, Supplier: Log in to see
Antigen
  • CD19
  • AW495831
  • B4
  • CVID3
  • spinster homolog 1 (Drosophila)
  • CD19 molecule
  • CD19 antigen
  • SPNS1
  • CD19
  • Cd19
Alternatives
anti-Human CD19 antibody for Immunoprecipitation
Reactivity
Human
1054
371
48
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5
3
3
Host
Mouse
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13
1
Clonality (Clone)
Monoclonal ()
Conjugate
This CD19 antibody is conjugated to PE
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96
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2
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1
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1
Application
Flow Cytometry (FACS), Immunoprecipitation (IP)
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Options
Supplier
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Supplier Product No.
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Immunogen Daudi human Burkitt lymphoma cell line
Clone LT19
Isotype IgG1
Specificity The antibody LT19 reacts with CD19 (B4), a 95 kDa type I transmembrane glycoprotein (immunoglobulin superfamily) expressed on B lymphocytes and follicular dendritic cells, it is lost on plasma cells.
HLDA 10
Characteristics The purified antibody is conjugated with R-Phycoerythrin (PE) under optimum conditions. The conjugate is purified by size-exclusion chromatography and adjusted for direct use. No reconstitution is necessary.
Alternative Name CD19 (CD19 Antibody Abstract)
Background CD19 is a transmembrane glycoprotein of Ig superfamily expressed by B cells from the time of heavy chain rearrangement until plasma cell differentiation. It forms a tetrameric complex with CD21 (complement receptor type 2), CD81 (TAPA-1) and Leu13. Together with BCR (B cell antigen receptor), this complex signals to decrease B cell treshold for activation by the antigen. Besides being signal-amplifying coreceptor for BCR, CD19 can also signal independently of BCR coligation and it turns out to be a central regulatory component upon which multiple signaling pathways converge. Mutation of the CD19 gene results in hypogammaglobulinemia, whereas CD19 overexpression causes B cell hyperactivity.
Research Area Stem Cells, Hematopoietic Progenitors, Hematopoietic Stem Cells, Adaptive Immunity, CD Antigens, Surface Receptors of Immune Cells
Pathways Fc-epsilon Receptor Signaling Pathway, EGFR Signaling Pathway, Neurotrophin Signaling Pathway
Application Notes The reagent is designed for Flow Cytometry analysis of human blood cells using 20 μ,l reagent / 100 μ,l of whole blood or 106 cells in a suspension.The content of a vial (2 ml) is sufficient for 100 tests.
Restrictions For Research Use only
Reconstitution No reconstitution is necessary.
Buffer The reagent is provided in phosphate buffered saline (PBS) containing 15 mM sodium azide and 0.2 % (w/v) high-grade protease free Bovine Serum Albumin (BSA) as a stabilizing agent.
Handling Advice Do not freeze.
Avoid prolonged exposure to light.
Storage 4 °C
Storage Comment Store in the dark at 2-8°C. Do not freeze. Avoid prolonged exposure to light. Do not use after expiration date stamped on vial label.
Supplier Images
Flow Cytometry (FACS) image for anti-CD19 Molecule (CD19) antibody (PE) (ABIN94016) Surface staining of human peripheral blood cells with anti-human CD19 (LT19) APC.
Product cited in: Kayserova, Vcelakova, Stechova, Dudkova, Hromadkova, Sumnik, Kolouskova, Spisek, Sediva: "Decreased dendritic cell numbers but increased TLR9-mediated interferon-alpha production in first degree relatives of type 1 diabetes patients." in: Clinical immunology (Orlando, Fla.), Vol. 153, Issue 1, pp. 49-55, 2014 (PubMed).

Elias, Flo, Lopez, Zorzopulos, Montaner, Rodriguez: "Strong cytosine-guanosine-independent immunostimulation in humans and other primates by synthetic oligodeoxynucleotides with PyNTTTTGT motifs." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 171, Issue 7, pp. 3697-704, 2003 (PubMed).

Background publications Svensson: "Isolation and culture of human hematopoietic progenitors for studies of dendritic cell biology." in: Methods in molecular biology (Clifton, N.J.), Vol. 531, pp. 187-202, 2009 (PubMed).

Gonçalves, Castro, Henriques, Oliveira, Pinheiro, Oliveira, Sreenu, Evans, Davis, Moreira, Carmo: "Molecular cloning and analysis of SSc5D, a new member of the scavenger receptor cysteine-rich superfamily." in: Molecular immunology, Vol. 46, Issue 13, pp. 2585-96, 2009 (PubMed).

Rayment, Kooij, Zhang, Siebold, Murphy, Allen, Willcox, Roberts: "Evidence for the specificity for platelet HPA-1a alloepitope and the presenting HLA-DR52a of diverse antigen-specific helper T cell clones from alloimmunized mothers." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 183, Issue 1, pp. 677-86, 2009 (PubMed).

Shao, Suresh, Vakil, Gomer, Pilling: "Pivotal Advance: Th-1 cytokines inhibit, and Th-2 cytokines promote fibrocyte differentiation." in: Journal of leukocyte biology, Vol. 83, Issue 6, pp. 1323-33, 2008 (PubMed).

Allen, Pang, Skowera, Ellis, Rackham, Lozanoska-Ochser, Tree, Leslie, Tremble, Dayan, Peakman: "Plasmacytoid dendritic cells are proportionally expanded at diagnosis of type 1 diabetes and enhance islet autoantigen presentation to T-cells through immune complex capture." in: Diabetes, Vol. 58, Issue 1, pp. 138-45, 2008 (PubMed).

Barat, Gilbert, Imbeault, Tremblay: "Extracellular ATP reduces HIV-1 transfer from immature dendritic cells to CD4+ T lymphocytes." in: Retrovirology, Vol. 5, pp. 30, 2008 (PubMed).

Harrison, Franklin, Campbell: "Enumeration of blood dendritic cells in patients with multiple myeloma at presentation and through therapy." in: Leukemia & lymphoma, Vol. 49, Issue 12, pp. 2272-83, 2008 (PubMed).

Vantourout, Martinez, Fabre, Collet, Champagne: "Ecto-F1-ATPase and MHC-class I close association on cell membranes." in: Molecular immunology, Vol. 45, Issue 2, pp. 485-92, 2007 (PubMed).

Shi, Xie, Chang, Zhou, Tedder, Mohan: "CD19 hyperexpression augments Sle1-induced humoral autoimmunity but not clinical nephritis." in: Arthritis and rheumatism, Vol. 56, Issue 9, pp. 3057-69, 2007 (PubMed).

Inabe, Kurosaki: "Tyrosine phosphorylation of B-cell adaptor for phosphoinositide 3-kinase is required for Akt activation in response to CD19 engagement." in: Blood, Vol. 99, Issue 2, pp. 584-9, 2002 (PubMed).

Fujimoto, Poe, Jansen, Sato, Tedder: "CD19 amplifies B lymphocyte signal transduction by regulating Src-family protein tyrosine kinase activation." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 162, Issue 12, pp. 7088-94, 1999 (PubMed).

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